Claire Duvallet

TL;DR: QIIME 2 development was primarily funded by NSF Awards 1565100 to J.G.C. and R.K.P. and partial support was also provided by the following: grants NIH U54CA143925 and U54MD012388.

TL;DR: QIIME 2 provides new features that will drive the next generation of microbiome research, including interactive spatial and temporal analysis and visualization tools, support for metabolomics and shotgun metagenomics analysis, and automated data provenance tracking to ensure reproducible, transparent microbiome data science.

TL;DR: The MicrobiomeHD database, which includes 28 published case–control gut microbiome studies spanning ten diseases, is introduced, and a cross-disease meta-analysis of these studies using standardized methods finds consistent patterns characterizing disease-associated microbiome changes.

TL;DR: A laboratory protocol to quantify viral titers in raw sewage via qPCR analysis and validate results with sequencing analysis suggests that the number of positive cases estimated from wastewater viral titer is orders of magnitude greater than the numberof confirmed clinical cases and therefore may significantly impact efforts to understand the case fatality rate and progression of disease.

TL;DR: Wastewater surveillance at a major urban treatment facility in Massachusetts found the presence of SARS-CoV-2 at high titers in the period from March 18 - 25 using RT-qPCR, and the identity of the PCR product was confirmed by direct DNA sequencing.