G
Grant A. Challen
Researcher at Washington University in St. Louis
Publications - 83
Citations - 6032
Grant A. Challen is an academic researcher from Washington University in St. Louis. The author has contributed to research in topics: Haematopoiesis & Stem cell. The author has an hindex of 27, co-authored 71 publications receiving 4853 citations. Previous affiliations of Grant A. Challen include Monash University, Clayton campus & Baylor University.
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Journal ArticleDOI
Dnmt3a is essential for hematopoietic stem cell differentiation.
Grant A. Challen,Deqiang Sun,Mira Jeong,Min Luo,Jaroslav Jelinek,Jonathan S. Berg,Jonathan S. Berg,Christoph Bock,Aparna Vasanthakumar,Hongcang Gu,Yuanxin Xi,Shoudan Liang,Yue Lu,Gretchen J. Darlington,Alexander Meissner,Jean Pierre J. Issa,Lucy A. Godley,Wei Li,Margaret A. Goodell +18 more
TL;DR: Using conditional ablation, it is shown that Dnmt3a loss progressively impairs hematopoietic stem cell differentiation over serial transplantation, while simultaneously expanding HSC numbers in the bone marrow.
Journal ArticleDOI
Distinct Hematopoietic Stem Cell Subtypes Are Differentially Regulated by TGF-β1
Grant A. Challen,Nathan C. Boles,Nathan C. Boles,Stuart M. Chambers,Stuart M. Chambers,Margaret A. Goodell,Margaret A. Goodell +6 more
TL;DR: This study demonstrates definitive isolation of lineage-biased HSC subtypes and contributes to the fundamental change in view that the hematopoietic system is maintained by a continuum of H SC subtypes, rather than a functionally uniform pool.
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Clonal haematopoiesis harbouring AML-associated mutations is ubiquitous in healthy adults
TL;DR: Methods for targeted error-corrected sequencing are developed, which enable the accurate detection of clonal mutations as rare as 0.0003 VAF, and observed clonal haematopoiesis, frequently harbouring mutations in DNMT3A and TET2, in 95% of individuals studied.
Journal ArticleDOI
A side order of stem cells: the SP phenotype.
TL;DR: The SP phenotype may prove invaluable for the initial isolation of resident tissue stem cells in the absence of definitive cell‐surface markers and may have broad‐ranging applications in stem cell biology, from the purification of novel stem cell populations to the development of autologous stem cell therapies.
Journal ArticleDOI
Mouse Hematopoietic Stem Cell Identification And Analysis
TL;DR: Different hematopoietic stem and progenitor purification strategies are reviewed and flow cytometry profiles for HSC sorting and analysis on different instruments are compared and novel strategies for working with rare cell populations such as HSCs are discussed.