J
Jonghyeon Shin
Researcher at Massachusetts Institute of Technology
Publications - 18
Citations - 2425
Jonghyeon Shin is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Protein biosynthesis & Sigma factor. The author has an hindex of 12, co-authored 17 publications receiving 2037 citations. Previous affiliations of Jonghyeon Shin include Sungkyunkwan University & University of Minnesota.
Papers
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Journal ArticleDOI
Genetic circuit design automation
Alec A. K. Nielsen,Bryan S. Der,Bryan S. Der,Jonghyeon Shin,Prashant Vaidyanathan,Vanya Paralanov,Elizabeth A. Strychalski,David J. Ross,Douglas Densmore,Christopher A. Voigt +9 more
TL;DR: Electronic design automation principles from EDA are applied to enable increased circuit complexity and to simplify the incorporation of synthetic gene regulation into genetic engineering projects, and it is demonstrated that engineering principles can be applied to identify and suppress errors that complicate the compositions of larger systems.
Journal ArticleDOI
An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells
Jonghyeon Shin,Vincent Noireaux +1 more
TL;DR: The construction and the phenomenological characterization of synthetic gene circuits engineered with a cell-free expression toolbox that works with the seven E. coli sigma factors are reported, revealing the importance of the global mRNA turnover rate and of passive competition-induced transcriptional regulation.
Journal ArticleDOI
Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology.
Zachary Z. Sun,Clarmyra A. Hayes,Jonghyeon Shin,Filippo Caschera,Richard M. Murray,Vincent Noireaux +5 more
TL;DR: The preparation and execution of an efficient endogenous E. coli based transcription-translation (TX-TL) cell-free expression system that can produce equivalent amounts of protein as T7-based systems at a 98% cost reduction to similar commercial systems is described.
Journal ArticleDOI
Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70.
Jonghyeon Shin,Vincent Noireaux +1 more
TL;DR: Although it uses the endogenous E. coli transcription machinery, this cell-free system can produce active proteins in quantities comparable to bacteriophage systems and provides much more possibilities to engineer informational processes in vitro.
Journal ArticleDOI
Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction
TL;DR: The demonstration that genome-sized viral DNA can be expressed in a test tube, recapitulating the entire chain of information processing including the replication of the DNA instructions, opens new possibilities to program and to study complex biochemical systems in vitro.