Luz-Maria Guzman

TL;DR: The tight regulation of the PBAD promoter is exploited to study the phenotypes of null mutations of essential genes and the use of pBAD vectors as an expression system is explored.

TL;DR: Under certain growth conditions, depletion of FtsL or expression of the largest ftsL-phoA fusion produced a variety of cell morphologies, including Y-shaped bacteria, indicating a possible general weakening of the cell wall.

TL;DR: It is concluded that immunofluorescence microscopy can be used to localize proteins whose abundance is as low as approximately 100 molecules per cell and that spatial and temporal regulation of FtsI activity in septum formation is achieved, at least in part, by timed localization of the protein to the division site.

TL;DR: The ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system and demonstrates the utility of this technology for rapidly discovering genes that affect the fitness of E. coli under a variety of growth conditions.

TL;DR: It is shown here that the same internal domain that promotes an inefficient translocation of murine PAI-2 in mammalian cells is a weak signal sequence in Escherichia coli, and mutations that improve the activity of the PAi-2 signal sequence and that convert the N-terminal regions of maspin and PI-10 into efficient signal sequences have been characterized.