O
Olivier Van Wuytswinkel
Researcher at University of Picardie Jules Verne
Publications - 21
Citations - 2392
Olivier Van Wuytswinkel is an academic researcher from University of Picardie Jules Verne. The author has contributed to research in topics: Gene & Linum. The author has an hindex of 14, co-authored 21 publications receiving 2161 citations. Previous affiliations of Olivier Van Wuytswinkel include Institut national de la recherche agronomique.
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Journal ArticleDOI
The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants
Laurent Gutierrez,Mélanie Mauriat,Stéphanie Guénin,Jérôme Pelloux,Jean-François Lefebvre,Romain Louvet,Christine Rusterucci,Thomas Moritz,François Guerineau,Catherine Bellini,Catherine Bellini,Olivier Van Wuytswinkel +11 more
TL;DR: Evidence is presented indicating that no gene can act as a universal reference, implying the need for a systematic validation of reference genes.
Journal ArticleDOI
Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references
Stéphanie Guénin,Mélanie Mauriat,Jérôme Pelloux,Olivier Van Wuytswinkel,Catherine Bellini,Catherine Bellini,Laurent Gutierrez,Laurent Gutierrez +7 more
TL;DR: The concept of normalization in transcript quantification is introduced here in an attempt to convince molecular biologists, and non-specialists, that systematic validation of reference genes is essential for producing accurate, reliable data in qRT-PCR analyses, and thus should be an integral component of them.
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Combined networks regulating seed maturation.
Laurent Gutierrez,Olivier Van Wuytswinkel,Mathieu Castelain,Catherine Bellini,Catherine Bellini +4 more
TL;DR: Seed maturation is an important phase of seed development during which embryo growth ceases, storage products accumulate, the protective tegument differentiates and tolerance to desiccation develops, leading to seed dormancy.
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Cellular and molecular aspects of iron metabolism in plants
Jean-François Briat,Isabelle Fobis-Loisy,Nicole Grignon,Stéphane Lobréaux,Nadine Pascal,Gil Savino,Séverine Thoiron,Nicolaus von Wirén,Olivier Van Wuytswinkel +8 more
TL;DR: Plant hormones such as auxin, abscisic acid and ethylene, as well as reactive oxygen intermediates play an important role in the transduction pathways, allowing plants to respond to these iron‐deficiency and excess stresses.
Journal ArticleDOI
Towards a Systematic Validation of References in Real-Time RT-PCR
TL;DR: Real-time RT-PCR is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection.