R
R. Boom
Researcher at University of Amsterdam
Publications - 28
Citations - 6939
R. Boom is an academic researcher from University of Amsterdam. The author has contributed to research in topics: Polymerase chain reaction & DNA. The author has an hindex of 21, co-authored 28 publications receiving 6749 citations.
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Journal ArticleDOI
Rapid and simple method for purification of nucleic acids.
R. Boom,C. J. A. Sol,M. M. M. Salimans,C. L. Jansen,P.M.E. Wertheim-van Dillen,J. van der Noordaa +5 more
TL;DR: A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.
Journal ArticleDOI
Development of virus-specific CD4+ T cells during primary cytomegalovirus infection
R.J Rentenaar,Laila E. Gamadia,Nicolette van derHoek,Frank N. J. Van Diepen,R. Boom,J. F. L. Weel,Pauline M. E. Wertheim-van Dillen,René A. W. van Lier,Ineke J. M. ten Berge +8 more
TL;DR: In this article, the authors analyzed the kinetics and characteristics of cytomegalovirus-specific (CMV-specific) CD4+ T cells in the course of primary CMV infection in kidney transplant recipients.
Journal ArticleDOI
Improved Silica-Guanidiniumthiocyanate DNA Isolation Procedure Based on Selective Binding of Bovine Alpha-Casein to Silica Particles
R. Boom,C. J. A. Sol,Marcel Beld,J. F. L. Weel,Jaap Goudsmit,Pauline M. E. Wertheim-van Dillen +5 more
TL;DR: In this paper, a novel lysis buffer containing alpha-casein was used for DNA purification in clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure.
Journal ArticleDOI
Rapid purification of hepatitis B virus DNA from serum.
TL;DR: It is shown, by Southern blot analysis, that HBV DNA could be reproducibly purified from human serum with the same yields by either procedure (30 to 50% relative to a classic procedure) and apparently independent of serum composition.
Journal ArticleDOI
Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription-PCR with an Armored RNA Internal Control
Marcel Beld,René Minnaar,J. F. L. Weel,C. J. A. Sol,Marjolein Damen,Harry van der Avoort,Pauline M. E. Wertheim-van Dillen,Alex van Breda,R. Boom +8 more
TL;DR: In this paper, a diagnostic reverse transcription (RT)-PCR was developed for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA.