■ Abstract Apoptosis is a genetically programmed, morphologically distinct form of cell death that can be triggered by a variety of physiological and pathological stimuli. Studies performed over the past 10 years have demonstrated that proteases play critical roles in initiation and execution of this process. The caspases, a family of cysteine-dependent aspartate-directed proteases, are prominent among the death proteases. Caspases are synthesized as relatively inactive zymogens that become activated by scaffold-mediated transactivation or by cleavage via upstream proteases in an intracellular cascade. Regulation of caspase activation and activity occurs at several different levels: ( a) Zymogen gene transcription is regulated; ( b) antiapoptotic members of the Bcl-2 family and other cellular polypeptides block proximity-induced activation of certain procaspases; and ( c) certain cellular inhibitor of apoptosis proteins (cIAPs) can bind to and inhibit active caspases. Once activated, caspases cleave a variety of intracellular polypeptides, including major structural elements of the cytoplasm and nucleus, components of the DNA repair machinery, and a number of protein kinases. Collectively, these scissions disrupt survival pathways and disassemble important architectural components of the cell, contributing to the stereotypic morphological and biochemical changes that characterize apoptotic cell death.

Mammalian caspases: structure ,a ctivation ,s ubstrates, and functions during apoptosis

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Methods Of Enzymatic Analysis

Myoglobin and lipid oxidation interactions: mechanistic bases and control

Fibre concentrates from apple pomace and citrus peel as potential fibre sources for food enrichment

A structural approach to understanding the interactions between colour, water-holding capacity and tenderness.

Effects of dry-aging on meat quality attributes and metabolite profiles of beef loins

The impacts of accelerated pH decline combined with high muscle temperature on post-mortem muscle metabolism and subsequent meat quality attributes have been extensively studied. Traditionally, this phenomenon has been observed in pork muscles, primarily due to the relatively fast post-mortem glycolysis rate and its relationships to stress susceptibility of pigs before slaughter. However, the protein-denaturing condition of high temperature/rapid pH fall and subsequent PSE (pale, soft and exudative)-like abnormal meat quality characteristics have been observed in muscles from other species such as beef, lamb, venison and even poultry. Various pre-rigor conditions including the application of electrical stimulation, hot-boning, and/or pre-rigor carcass chilling temperatures in various muscles, in conjunction with carcass stretching/hanging methods, can also contribute to muscle-protein denaturation pre-rigor. This review considers the influence of a faster than normal pH fall at a higher than normal pre-rigor temperature on glycolysis, post-mortem muscle proteins and subsequently meat quality attributes. Gaps in current knowledge are identified and recommendations made for additional research.

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Influence of high pre-rigor temperature and fast pH fall on muscle proteins and meat quality: a review

The objective of this study was to determine the effects of adding pre-treated mealworm larvae ( Tenebrio molitor ) and silkworm pupae ( Bombyx mori ) flours on nutritional, physicochemical and textural properties of emulsion sausages. Whole freeze-dried insects were sequentially ground, defatted, and acid-hydrolyzed. Control sausage was formulated with 60% lean pork, 20% ice and 20% back fat, and insect treatments were prepared with replacement of 10% lean pork by each pre-treated insect flour. Defatting and/or acid hydrolysis significantly increased the protein content of two insect flours, but acid hydrolysis slightly decreased protein solubility ( P  = 0.002). The addition of pre-treated insect flours had no impact on protein solubility of emulsion sausages, but increased cooking yield and hardness in a similar extent, regardless of pre-treating methods and insect types ( P  > 0.05). Our results suggest that through separation processing, mealworm larvae and silkworm pupae can be further optimized as a novel protein ingredient for emulsified meat products. Industrial Relevance This study evaluated the nutritional and technological properties of pre-treated edible insects as a novel non-meat ingredient for the meat emulsion application. The results of the present study suggest that edible insect proteins can be practically utilized as a non-meat food ingredient in processed meat products or potentially other food applications without compromising the nutritional and technological properties of the products.

Yuan H. Brad Kim

Papers

Effects of dry-aging on meat quality attributes and metabolite profiles of beef loins

Influence of high pre-rigor temperature and fast pH fall on muscle proteins and meat quality: a review

Pre-treated mealworm larvae and silkworm pupae as a novel protein ingredient in emulsion sausages

Understanding postmortem biochemical processes and post-harvest aging factors to develop novel smart-aging strategies.

Evaluation of combined effects of ageing period and freezing rate on quality attributes of beef loins.