Singapore–MIT alliance

About: Singapore–MIT alliance is a based out in . It is known for research contribution in the topics: Finite element method & Smoothed finite element method. The organization has 1243 authors who have published 2381 publications receiving 77172 citations.

Tuning upconversion through energy migration in core–shell nanoparticles

Abstract: Photon upconversion is promising for applications such as biological imaging, data storage or solar cells. Here, we have investigated upconversion processes in a broad range of gadolinium-based nanoparticles of varying composition. We show that by rational design of a core-shell structure with a set of lanthanide ions incorporated into separated layers at precisely defined concentrations, efficient upconversion emission can be realized through gadolinium sublattice-mediated energy migration for a wide range of lanthanide activators without long-lived intermediary energy states. Furthermore, the use of the core-shell structure allows the elimination of deleterious cross-relaxation. This effect enables fine-tuning of upconversion emission through trapping of the migrating energy by the activators. Indeed, the findings described here suggest a general approach to constructing a new class of luminescent materials with tunable upconversion emissions by controlled manipulation of energy transfer within a nanoscopic region.

Upconversion nanoparticles in biological labeling, imaging, and therapy

Abstract: Upconversion refers to non-linear optical processes that convert two or more low-energy pump photons to a higher-energy output photon. After being recognized in the mid-1960s, upconversion has attracted significant research interest for its applications in optical devices such as infrared quantum counter detectors and compact solid-state lasers. Over the past decade, upconversion has become more prominent in biological sciences as the preparation of high-quality lanthanide-doped nanoparticles has become increasingly routine. Owing to their small physical dimensions and biocompatibility, upconversion nanoparticles can be easily coupled to proteins or other biological macromolecular systems and used in a variety of assay formats ranging from bio-detection to cancer therapy. In addition, intense visible emission from these nanoparticles under near-infrared excitation, which is less harmful to biological samples and has greater sample penetration depths than conventional ultraviolet excitation, enhances their prospects as luminescent stains in bio-imaging. In this article, we review recent developments in optical biolabeling and bio-imaging involving upconversion nanoparticles, simultaneously bringing to the forefront the desirable characteristics, strengths and weaknesses of these luminescent nanomaterials.

A Smoothed Finite Element Method for Mechanics Problems

Abstract: In the finite element method (FEM), a necessary condition for a four-node isoparametric element is that no interior angle is greater than 180° and the positivity of Jacobian determinant should be ensured in numerical implementation. In this paper, we incorporate cell-wise strain smoothing operations into conventional finite elements and propose the smoothed finite element method (SFEM) for 2D elastic problems. It is found that a quadrilateral element divided into four smoothing cells can avoid spurious modes and gives stable results for integration over the element. Compared with original FEM, the SFEM achieves more accurate results and generally higher convergence rate in energy without increasing computational cost. More importantly, as no mapping or coordinate transformation is involved in the SFEM, its element is allowed to be of arbitrary shape. Hence the restriction on the shape bilinear isoparametric elements can be removed and problem domain can be discretized in more flexible ways, as demonstrated in the example problems.

Isolation and retrieval of circulating tumor cells using centrifugal forces

Abstract: Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5–88 CTCs per mL). The presence of CD133+ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.