Journal ArticleDOI
A rapid DNA isolation procedure for small quantities of fresh leaf tissue
Edwin Pahlich,Chr. Gerlitz +1 more
TLDR
From the kinetic data, it becomes evident that the reductive amination reaction is highly adaptive to the ammonium environment.About:
This article is published in Phytochemistry.The article was published on 1980-01-01. It has received 14480 citations till now. The article focuses on the topics: Ammonium & Substrate (chemistry).read more
Citations
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A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species
Robert J. Elshire,Jeffrey C. Glaubitz,Qi-ying Sun,Jesse Poland,Ken Kawamoto,Edward S. Buckler,Edward S. Buckler,Sharon E. Mitchell +7 more
TL;DR: A procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs) is reported, which is simple, quick, extremely specific, highly reproducible, and may reach important regions of the genome that are inaccessible to sequence capture approaches.
Journal ArticleDOI
Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components
TL;DR: This is the first procedure for the isolation of DNA from mature strawberry leaf tissue that produces consistent results for a variety of different species, both octoploid and diploid, and is both stable and PCR amplifiable before and after extended storage.
Journal ArticleDOI
Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: the tortoise and the hare III
TL;DR: Nine newly explored regions of the chloroplast genome offer levels of variation better than the best regions identified in an earlier study and are therefore likely to be the best choices for molecular studies at low taxonomic levels.
Journal ArticleDOI
The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis
Joey Shaw,Edgar B. Lickey,John T. Beck,Susan B. Farmer,Wusheng Liu,Jermey Miller,Kunsiri Chaw Siripun,Charles Thomas Winder,Edward E. Schilling,Randall L. Small +9 more
TL;DR: The results of this study show that a survey using as few as three representative taxa can be predictive of the amount of phylogenetic information offered by a cpDNA region and that rate heterogeneity exists among noncoding cpDNA regions.
Journal ArticleDOI
Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme Genotyping-by-Sequencing Approach
Jesse Poland,Jesse Poland,Patrick J. Brown,Mark E. Sorrells,Jean-Luc Jannink,Jean-Luc Jannink +5 more
TL;DR: The GBS approach presented here provides a powerful method of developing high-density markers in species without a sequenced genome while providing valuable tools for anchoring and ordering physical maps and whole-genome shotgun sequence.
References
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Journal ArticleDOI
The pathway of nitrogen assimilation in plants
Benjamin J. Miflin,Peter J. Lea +1 more
TL;DR: There is other evidence to support the contention that the assimilation of ammonia into amino acids occurs via glutamine synthetase and glutamate synthase, and that it is unlikely that glutamate dehydrogenase plays a major role in nitrogen assimilation in bacteria or higher plants except in circumstances of ammonia excess.
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Kinetic studies of glutamate dehydrogenase with glutamate and norvaline as substrates. Coenzyme activation and negative homotropic interactions in allosteric enzymes.
Paul C. Engel,Keith Dalziel +1 more
TL;DR: It is suggested that sharp discontinuities in Lineweaver-Burk plots or reciprocal binding plots may be characteristic of this new type of interaction, which can be explained in terms of an Adair-Koshland model, but not by the model of Monod, Wyman & Changeux.
Journal ArticleDOI
The isolation and characterization of glutamic dehydrogenase from corn leaves
TL;DR: The partially purified glutamic dehydrogenase from corn leaves has a pH optimum of 8.1, a coenzyme specificity for DPN, and a substrate specificity for l -glutamate, and was not inhibited by cyanide, hydroxy acids, or o-iodosobenzoate.
Journal ArticleDOI
Studies of glutamate dehydrogenase. The influence of ADP, GTP, and L-glutamate on the binding of the reduced coenzyme to beef-liver glutamate dehydrogenase.
Rudolf Koberstein,Horst Sund +1 more
TL;DR: The detailed investigation of the binding of NAD(P)H to beef liver glutamate dehydrogenase in the presence of the effectors ADP and GTP shows that each oligomer has a total of I8 nucleotide binding sites, and glutamate induces strong positive cooperativity of three of the active sites within the oligomer characterized by a Hill coefficient.