Journal ArticleDOI
Comparative release of growth factors from PRP, PRF, and advanced-PRF
Eizaburo Kobayashi,Laura Flückiger,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Kosaku Sawada,Kosaku Sawada,Anton Sculean,Benoit Schaller,Richard J. Miron +8 more
TLDR
PRP can be recommended for fast delivery of growth factors whereas A-PRF is better-suited for long-term release, according to the results of the present study.Abstract:
The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF). Eighteen blood samples were collected from six donors (3 samples each for PRP, PRF, and A-PRF). Following preparation, samples were incubated in a plate shaker and assessed for growth factor release at 15 min, 60 min, 8 h, 1 day, 3 days, and 10 days. Thereafter, growth factor release of PDGF-AA, PDGF-AB, PDGF-BB, TGFB1, VEGF, EGF, and IGF was quantified using ELISA. The highest reported growth factor released from platelet concentrates was PDGF-AA followed by PDGF-BB, TGFB1, VEGF, and PDGF-AB. In general, following 15–60 min incubation, PRP released significantly higher growth factors when compared to PRF and A-PRF. At later time points up to 10 days, it was routinely found that A-PRF released the highest total growth factors. Furthermore, A-PRF released significantly higher total protein accumulated over a 10-day period when compared to PRP or PRF. The results from the present study indicate that the various platelet concentrates have quite different release kinetics. The advantage of PRP is the release of significantly higher proteins at earlier time points whereas PRF displayed a continual and steady release of growth factors over a 10-day period. Furthermore, in general, it was observed that the new formulation of PRF (A-PRF) released significantly higher total quantities of growth factors when compared to traditional PRF. Based on these findings, PRP can be recommended for fast delivery of growth factors whereas A-PRF is better-suited for long-term release.read more
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Journal ArticleDOI
Optimized Platelet-Rich Fibrin With the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response
Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Richard J. Miron,Maria Hernandez,Umadevi Kandalam,Yufeng Zhang,Joseph Choukroun +7 more
TL;DR: Modifications to centrifugation speed and time with the low-speed concept favor an increase in growth factor release from PRF clots, which may directly influence tissue regeneration by increasing fibroblast migration, proliferation, and collagen mRNA levels.
Journal ArticleDOI
Use of platelet-rich fibrin in regenerative dentistry: a systematic review.
Richard J. Miron,Richard J. Miron,Giovanni Zucchelli,Michael A. Pikos,Maurice Salama,Samuel Lee,Vincent Guillemette,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Mark Bishara,Yufeng Zhang,Hom-Lay Wang,Fatiha Chandad,Cleopatra Nacopoulos,Alain Simonpieri,Alexandre Amir Aalam,Pietro Felice,Gilberto Sammartino,Shahram Ghanaati,Maria Hernandez,Joseph Choukroun +21 more
TL;DR: Overall, the use of PRF has been most investigated in periodontology for the treatment of periodontal intrabony defects and gingival recessions where the majority of studies have demonstrated favorable results in soft tissue management and repair.
Journal ArticleDOI
Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?
Richard J. Miron,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Maria Hernandez,Umadevi Kandalam,Yufeng Zhang,Shahram Ghanaati,Joseph Choukroun +8 more
TL;DR: The findings from the present study demonstrate that a potent formulation of liquid platelet concentrates could be obtained without use of anti-coagulants and demonstrate the ability to release higher concentrations of various growth factors and induced higher fibroblast migration and expression of PDGF, TGF-β, and collagen1.
Journal ArticleDOI
Platelet-Rich Fibrin and Soft Tissue Wound Healing: A Systematic Review
Richard J. Miron,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Masako Fujioka-Kobayashi,Mark Bishara,Yufeng Zhang,Maria Hernandez,Joseph Choukroun +7 more
TL;DR: In this paper, a systematic review gathered all the currently available in vitro, in vivo, and clinical literature utilizing platelet-rich fibrin (PRF) for soft tissue regeneration, augmentation, and wound healing.
Journal ArticleDOI
Advances in surgical applications of growth factors for wound healing
Sho Yamakawa,Kenji Hayashida +1 more
TL;DR: Current approved growth factor therapies are emphasized, including a sustained release system for growth factors, emerging therapies, and future research possibilities combined with surgical procedures, to provide definitive confirmation of the efficacy of growth factors for intractable wounds.
References
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Journal ArticleDOI
Platelet-rich plasma: Growth factor enhancement for bone grafts
Robert E. Marx,Eric R. Carlson,Ralph M. Eichstaedt,Ralph M. Eichstaedt,Steven R Schimmele,Steven R Schimmele,James E. Strauss,Karen R. Georgeff +7 more
TL;DR: Monoclonal antibody assessment of cancellous cellular marrow grafts demonstrated cells that were capable of responding to the growth factors by bearing cell membrane receptors and evidenced a radiographic maturation rate 1.62 to 2.16 times that of grafts without platelet-rich plasma.
Journal ArticleDOI
Platelet-rich plasma: evidence to support its use
TL;DR: Not all currently marketed PRP devices are qual; some do not concentrate viably active platelets n sufficient numbers to produce a healing enhance- herefore, the term PRP is preferred to autologous latelet gel, plasma-rich growth factors (PRGFs), or a ere autOLOGous platelet concentrate.
Journal ArticleDOI
Platelet-rich plasma (PRP): what is PRP and what is not PRP?
TL;DR: The definition of PRP, its safety, its proper development, and its most efficacious means of application are discussed.
Journal ArticleDOI
Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution.
D. Dohan,Joseph Choukroun,Antoine Diss,Steve L. Dohan,Anthony Dohan,Jaafar Mouhyi,Bruno Gogly +6 more
TL;DR: A retrospective analysis is necessary for the understanding of fibrin technologies and the evaluation of the biochemical properties of 3 generations of surgical additives, respectively fibr in adhesives, concentrated platelet-rich plasma (cPRP) and PRF.
Journal ArticleDOI
Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features.
D. Dohan,Joseph Choukroun,Antoine Diss,Steve L. Dohan,Anthony Dohan,Jaafar Mouhyi,Bruno Gogly +6 more
TL;DR: Initial analyses revealed that slow fibrin polymerization during PRF processing leads to the intrinsic incorporation of platelet cytokines and glycanic chains in the fibrIn meshes, which would imply that PRF, unlike the other platelet concentrates, would be able to progressively release cytokines during fibr in matrix remodeling; such a mechanism might explain the clinically observed healing properties of PRF.