Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities
TLDR
The Nelson-Somogyi and 3,5-dinitrosalicylic acid assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides, and reasons for preferential use of the NS assay for measuring activities of carbohydrases other than cellulases are discussed.Abstract:
The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities againstdifferent polysaccharides. Using twelve commercial enzyme preparations, the comparisonof the NS and DNS assays in determination of cellulase, β-glucanase, xylanase, and β-mannanase activities was carried out. When cellulase activities againstCMC were measured, the DNS assaygave activity values,whichwere typically 40–50%higherthanthoseobtained with the NS assay. In the analysis of the xylanase, β-mannanase, and β-glucanase activities, the overestimations by the DNS assay were much more pronounced (the observed differences in the activities were 3- to 13-fold). Reasons for preferential use of the NS assay for measuring activities of carbohydrases other than cellulases are discussed.read more
Citations
More filters
α-Amylase Production and Applications: A Review
TL;DR: The ubiquitous nature, ease of production and broad spectrum of applications make α-Amylase an industrially important enzyme.
Journal ArticleDOI
Biotechnological Processes in Microbial Amylase Production.
Subash C. B. Gopinath,Periasamy Anbu,M. K. Md Arshad,Thangavel Lakshmipriya,Chun Hong Voon,Uda Hashim,Suresh V. Chinni +6 more
TL;DR: The importance of microbial (bacteria and fungi) amylase is discussed along with its production methods from the laboratory to industrial scales.
Journal ArticleDOI
Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton
TL;DR: In this paper , the role of GhGLU19, a gene encoding β-1,3-glucanase, in cotton seed germination was examined, and the effect of callose on water uptake and following seed metabolic events during germination were largely unknown.
Journal ArticleDOI
Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements.
Ricardo Sposina Sobral Teixeira,Ayla Sant’Ana da Silva,Viridiana Santana Ferreira-Leitão,Elba P. S. Bon +3 more
TL;DR: It was found that in reaction mixtures containing 20mM of either tryptophan, cysteine, histidine, tyrosine, or hydroxyproline the measurement of 3.7 mM glucose was overestimated by 76%, 50%, 35%, 18%, and 10%, respectively, while methionine decreased the color development by 5%.
Journal ArticleDOI
Penicillium: The next emerging champion for cellulase production
Neha Vaishnav,Anusuiya Singh,Mukund Adsul,Pooja Dixit,Simranjeet Kaur Sandhu,Anshu S. Mathur,Suresh Kumar Puri,Reeta Rani Singhania +7 more
TL;DR: It is justified that Penicillium could be the next champions for cellulase production for biofuel applications because it gives higher efficiencies during saccharification of biomass resulting in lower cellulase requirement; decreasing the cost of cellulases in the process.
References
More filters
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
A photometric adaptation of the somogyi method for the determination of glucose
TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.
Journal ArticleDOI
Measurement of cellulase activities
TL;DR: Cooney et al. as mentioned in this paper proposed a new committee for the first time in 1981, with the following members: H. H. Cooney (USA), V. G. E. Ertola (Argentina; 1981-85); P. P. Stewart (Canada; Associate 1981-83); J. K. Jagannathan (India; 1983, 1985); L. C. Deliweg (FRG); 1983-85; G. G E. Righelato (UK); 1983, 85; and R. L. Davis (
Journal ArticleDOI
A classification of glycosyl hydrolases based on amino acid sequence similarities.
TL;DR: With the steady increase in sequence and structural data, it is suggested that the enzyme classification system should perhaps be revised.