Open AccessJournal Article
Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication
R Brun,Schönenberger +1 more
Reads0
Chats0
About:
This article is published in Acta Tropica.The article was published on 1979-09-01 and is currently open access. It has received 1143 citations till now. The article focuses on the topics: Trypanosoma brucei & Procyclin.read more
Citations
More filters
Journal ArticleDOI
A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei.
TL;DR: A dual-promoter approach is developed, for expressing highly toxic products and generating conditional gene knock-outs, using back-to-back constitutive T7 and tetracycline-responsive PARP promoters to drive expression of the selectable marker and test gene, respectively.
Journal ArticleDOI
A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue.
Judith Mikus,Dietmar Steverding +1 more
TL;DR: A quantitative colorimetric assay using the oxidation-reduction indicator Alamar Blue was developed to measure cytotoxicity of compounds against the protozoan parasite Leishmania and permits a simple, reproducible and reliable method for screening antileishmanial drugs.
Journal ArticleDOI
The cell division cycle of Trypanosoma brucei brucei: timing of event markers and cytoskeletal modulations.
Trevor Sherwin,Keith Gull +1 more
TL;DR: The timing and order of events occurring within the cell division cycle of Trypanosoma brucei has been derived from analyses of the proportion of a given cell type occurring witin an exponentially growing culture.
Journal ArticleDOI
Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei
R. Woodward,K. Gull +1 more
TL;DR: Immunofluorescent detection of 5-bromo-2-deoxyuridine-substituted DNA is used to determine the timing of initiation and the duration of nuclear and kinetoplast S-phases within the procyclic stage of the Trypanosoma brucei cell cycle and provides detection of the earliest known cell cycle event in T. Brucei.
Journal ArticleDOI
Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
T. Nicolai Siegel,Doeke R. Hekstra,Louise E. Kemp,Luisa M. Figueiredo,Joanna E. Lowell,David Fenyö,Xuning Wang,Scott Dewell,George A. M. Cross +8 more
TL;DR: It is suggested that histone modifications and histone variants play crucial roles in transcription initiation and termination in trypanosomes and that destabilization of nucleosomes by hist one variants is an evolutionarily ancient and general mechanism of transcription initiation, demonstrated in an organism in which general pol II transcription factors have been elusive.
Related Papers (5)
Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers.
Hiroyuki Hirumi,K. Hirumi +1 more
The Genome of the African Trypanosome Trypanosoma brucei
Matthew Berriman,Elodie Ghedin,Elodie Ghedin,Christiane Hertz-Fowler,Gaëlle Blandin,Hubert Renauld,Daniella Castanheira Bartholomeu,Nicola Lennard,Elisabet Caler,N. Hamlin,Brian J. Haas,Ulrike Böhme,Linda Hannick,Martin Aslett,Joshua Shallom,Lucio Marcello,Lihua Hou,Bill Wickstead,U. Cecilia M. Alsmark,Claire Arrowsmith,Rebecca Atkin,Andrew Barron,Frédéric Bringaud,Karen Brooks,Mark Carrington,Inna Cherevach,Tracey-Jane Chillingworth,Carol Churcher,Louise Clark,Craig Corton,Ann Cronin,Robert L. Davies,Jonathon Doggett,Appolinaire Djikeng,Tamara Feldblyum,Mark C. Field,Audrey Fraser,Ian Goodhead,Zahra Hance,David Harper,Barbara Harris,Heidi Hauser,Jessica B. Hostetler,Al Ivens,Kay Jagels,David W. Johnson,Justin Johnson,Kristine Jones,Arnaud Kerhornou,Hean Koo,Natasha Larke,Scott M. Landfear,Christopher Larkin,Vanessa Leech,Alexandra Line,Angela Lord,Annette MacLeod,P. Mooney,Sharon Moule,David M. A. Martin,Gareth W. Morgan,Karen Mungall,Halina Norbertczak,Doug Ormond,Grace Pai,Christopher S. Peacock,Jeremy Peterson,Michael A. Quail,Ester Rabbinowitsch,Marie-Adèle Rajandream,Chris P Reitter,Steven L. Salzberg,Mandy Sanders,Seth Schobel,Sarah Sharp,Mark Simmonds,Anjana J. Simpson,Luke J. Tallon,C. Michael R. Turner,Andrew Tait,Adrian Tivey,Susan Van Aken,Danielle Walker,David Wanless,Shiliang Wang,Brian White,Owen White,Sally Whitehead,John Woodward,Jennifer R. Wortman,Mark Raymond Adams,T. Martin Embley,Keith Gull,Elisabetta Ullu,J. David Barry,Alan H. Fairlamb,Fred R. Opperdoes,Barclay G. Barrell,John E. Donelson,Neil Hall,Neil Hall,Claire M. Fraser,Sara E. Melville,Najib M. El-Sayed,Najib M. El-Sayed +104 more