Journal ArticleDOI
Detection and quantification of fusarium culmorum and fusarium graminearum in cereals using pcr assays
TLDR
In this article, random amplified polymorphic DNA assays were used to identify amplification products characteristic of either Fusarium culmorum or fusarium graminearum and selected fragments were cloned, sequenced and primer pairs were developed which permitted specific detection of F. graminearlyum using conventional PCR.About:
This article is published in Physiological and Molecular Plant Pathology.The article was published on 1998-07-01. It has received 520 citations till now. The article focuses on the topics: Fusarium culmorum & Trichothecene.read more
Citations
More filters
Journal ArticleDOI
Molecular diagnostics for fungal plant pathogens
TL;DR: PCR-based methods can provide new tools to monitor the exposure of a crop to pathogen inoculum that are more reliable and faster than conventional methods that can be used to improve disease control decision making.
Journal ArticleDOI
Major changes in Fusarium spp. in wheat in the Netherlands
Cees Waalwijk,Pieter Kastelein,Ineke de Vries,Zoltán Kerényi,Theo van der Lee,Thamara Hesselink,Jürgen Köhl,Gert H. J. Kema +7 more
TL;DR: A diagnostic PCR method was developed to detect the most common species of Fusarium occurring on wheat, and demonstrated that F. graminearum was the most abundant species in the FUSarium complex on wheat in both years.
Journal ArticleDOI
Impacts of Molecular Diagnostic Technologies on Plant Disease Management
TL;DR: The application of ELISA and monoclonal antibody technologies in plant pathology has greatly improved the ability to detect plant pathogens and is increasing the understanding of, their ecology and epidemiology.
Journal ArticleDOI
Fusarium culmorum: causal agent of foot and root rot and head blight on wheat
Barbara Scherm,Virgilio Balmas,Francesca Spanu,Giovanna Pani,Giovanna Delogu,Matias Pasquali,Quirico Migheli +6 more
TL;DR: This review summarizes recent research activities related to F.’sculmorum, including studies into its population diversity, mycotoxin biosynthesis, mechanisms of pathogenesis and resistance, the development of diagnostic tools and preliminary genome sequence surveys, and proposed potential research areas that may expand the basic understanding of the wheat-F.
Journal ArticleDOI
Quantitative detection of Fusarium species in wheat using TaqMan
Cees Waalwijk,Ruth van der Heide,Ineke de Vries,Theo van der Lee,C.D. Schoen,Guillaume Costrel-de Corainville,Isolde Häuser-Hahn,Pieter Kastelein,Jürgen Köhl,Philippe Lonnet,Thierry Demarquet,Gert H. J. Kema +11 more
TL;DR: The Fusarium TaqMan technology is an important tool to quantify and monitor the dynamics of individual species of the complex causing FHB in cereals during the growing season.
References
More filters
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
A decimal code for the growth stages of cereals
TL;DR: The decimal code scale for indicating the growth stages of cereals as developed by the authors and published by Eucarpia is explained and described.