Dynamic light scattering: a practical guide and applications in biomedical sciences.
TLDR
Evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein–protein or protein–nucleic acid preparations, as well as to study protein–small molecule interactions is provided.Abstract:
Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein–protein or protein–nucleic acid preparations, as well as to study protein–small molecule interactions. Further, we provide examples of DLS’s application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii.read more
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References
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Uber einen die Erzeugung und Verwandlung des Lichtes betreffenden heuristischen Gesichtspunkt
TL;DR: In el marco del Proyecto subvencionado by the Fundación Antorchas (FAN) as mentioned in this paper, el material was digitalizado, e.g., en la Biblioteca del Departamento de Fisica de la Facultad de Ciencias Exactas de la Universidad Nacional de La Plata.
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Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of Cumulants
TL;DR: The first order electric field correlation function of laser light scattered by polydisperse solutions of macromolecules can be written as a sum or distribution of exponentials, with decay rates proportional to the diffusion coefficients of the solute molecules as discussed by the authors.