scispace - formally typeset
Journal ArticleDOI

Molecular Markers: It's Application in Crop Improvement

TLDR
Most of the available DNA markers that can be routinely employed in various aspects of plant genome analysis such as characterization of genetic variability, genome fingerprinting, genome mapping, gene localization, analysis of genome evolution, population genetics, taxonomy, plant breeding, and diagnostics are reviewed.
Abstract
Over the past few decades, plant genomics research has been studied extensively bringing about a revolution in the field of plant biotechnology. Molecular markers, useful for plant genome analysis, have now become an important tool in crop improvement. The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in their principles, methodologies and applications require careful consideration in choosing one or more of such methods. No molecular markers are available yet that fulfill all requirements needed by researchers. In this article we attempt to review most of the available DNA markers that can be routinely employed in various aspects of plant genome analysis such as characterization of genetic variability, genome fingerprinting, genome mapping, gene localization, analysis of genome evolution, population genetics, taxonomy, plant breeding, and diagnostics. The emerging patterns make up a unique feature of the analyzed individual and are currently considered to be the ultimate tool for biological individualization.

read more

Citations
More filters

Caracterización molecular de arveja arbustiva (Pisum sativum L) en la zona cerealista del departamento de Nariño, Colombia

Duque Zapata, +1 more
TL;DR: La arveja (Pisum sativum L.) es uno de los cultivos domesticados mas antiguos, altamente valorados y ampliamente cultivados en todo el mundo, en Colombia esta especie carece de estudios geneticos que permitan establecer la variabilidad total.
Journal ArticleDOI

RNA-Seq of Guar (Cyamopsis tetragonoloba, L. Taub.) Leaves: De novo Transcriptome Assembly, Functional Annotation and Development of Genomic Resources.

TL;DR: This is the first report on transcriptome analysis and SNPs identification in guar till date and identifies 145 polymorphic SSR markers in two varieties.
Journal ArticleDOI

Variability Assessment for Root and Drought Tolerance Traits and Genetic Diversity Analysis of Rice Germplasm using SSR Markers.

TL;DR: Diverse germplasm of NE India could be used for the improvement of root and drought tolerance in rice breeding programmes and is suggested to be an important step for improvements of abiotic and biotic stressolerance in rice.
Journal ArticleDOI

Genetic diversity and population structure of Bambara groundnut (Vigna subterranea (L.) Verdc.): synopsis of the past two decades of analysis and implications for crop improvement programmes

TL;DR: An overview of the past two decades of genetic diversity analysis of Bambara groundnut landraces is presented, finding high genetic diversity and low heterozygosity have been reported across the germplasm analysed in this highly cleistogamous species.
References
More filters
Journal ArticleDOI

DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI

AFLP: a new technique for DNA fingerprinting.

TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal Article

Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

TL;DR: A new basis for the construction of a genetic linkage map of the human genome is described, to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA.
Journal ArticleDOI

Fingerprinting genomes using PCR with arbitrary primers

TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Book

Molecular Markers, Natural History, and Evolution

John C. Avise
TL;DR: A history of Molecular Phylogenetics and applications of individuality and Parentage, issues of Heterozygosity, and special Approaches to Phylageny Estimation are reviewed.
Related Papers (5)