Journal ArticleDOI
Narrow Genetic Variability in Cicer arietinum L. as Revealed by RFLP Analysis
TLDR
A subgenomic library constructed from small Pstl restriction fragments yielded 83.18% low-copy clones and the RFLP analysis of chloroplast and mitochondrial genomes showed no polymorphism.Abstract:
A subgenomic library constructed from small Pstl restriction fragments (0.4 to 2.0 kb) yielded 83.18% low-copy clones. Using 17 random genomic and 5 heterologous probes in 65 probe-enzyme combinations, Restriction Fragment Length Polymorphism (RFLP) for nuclear DNA was studied in five desi and five kabuli type chickpea cultivars. Only two clones revealed polymorphism in the cultivars tested. No polymorphism in chickpea varieties was detected with four Random Amplified Polymorphic DNA (RAPD) markers studied. However, some degree of polymorphism between C. arietinum and its wild relative C. reticulatum was detected. The RFLP analysis of chloroplast and mitochondrial genomes showed no polymorphism.read more
Citations
More filters
Journal ArticleDOI
A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum×C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5
Peter Winter,Ana Maria Benko-Iseppon,Bruno Hüttel,M. Ratnaparkhe,A. Tullu,G. Sonnante,T. Pfaff,M. Tekeoglu,Dipak K. Santra,V. J. Sant,P.N. Rajesh,Guenter Kahl,Fred J. Muehlbauer +12 more
TL;DR: The integrated molecular marker map of the chickpea genome was established using 130 recombinant inbred lines from a wide cross between a cultivar resistant to fusarium wilt caused by Fusarium oxysporum Schlecht to serve as a basis for marker-assisted selection and map-based cloning of fusaria wilt resistance genes and other agronomically important genes in future.
Journal ArticleDOI
Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome.
Peter Winter,T. Pfaff,Sripada M. Udupa,Bruno Hüttel,Prakash C. Sharma,S. Sahi,R. Arreguin-Espinoza,F. Weigand,Fred J. Muehlbauer,Guenter Kahl +9 more
TL;DR: The present STMS marker map represents the most advanced co-dominant DNA marker map of the chickpea genome and was genetically mapped in 90 recombinant inbred lines from an inter-species cross between C. reticulatum and C. echinospermum.
Journal ArticleDOI
Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.).
TL;DR: Two small-insert genomic libraries of chickpea were screened with a set of microsatellite-specific oligonucleotide probes and Mendelian inheritance of CaSTMS markers was demonstrated using aSet of recombinant inbred lines and their parents.
Journal ArticleDOI
Transcriptional profiling of chickpea genes differentially regulated in response to high-salinity, cold and drought
TL;DR: The annotation of genes from a set of previously identified putative stress-responsive genes from chickpea suggest that they may have a role in abiotic stress response and are potential candidates for tolerance/susceptibility.
Journal ArticleDOI
The origins of cultivation of Cicer arietinum L. and Vicia faba L.: early finds from Tell el-Kerkh, north-west Syria, late 10th millennium b.p.
Ken-ichi Tanno,George Willcox +1 more
TL;DR: The finds of C. arietinum and Vicia faba suggest that the use and domestication of these pulses is perhaps earlier than was previously supposed.
References
More filters
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Book
Basic methods in molecular biology
TL;DR: General methods bacterial strains and cloning vectors enzymes that modify DNA and RNA in vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction DNA restriction fragment analysis and preparation.
Journal ArticleDOI
A second-generation linkage map of the human genome.
Jean Weissenbach,Gabor Gyapay,Colette Dib,Alain Vignal,Jean Morissette,Philippe Millasseau,Guy Vaysseix,Mark Lathrop,Mark Lathrop +8 more
TL;DR: A linkage map of the human genome has been constructed based on the segregation analysis of 814 newly characterized polymorphic loci containing short tracts of (C-A)n repeats in a panel of DNAs from eight large families.
Journal ArticleDOI
Molecular mapping of rice chromosomes.
Susan R. McCouch,G. Kochert,Z. H. Yu,Z. Y. Wang,Gurdev S. Khush,W. R. Coffman,Steven D. Tanksley +6 more
TL;DR: It is discovered that rice DNA is less C-methylated than tomato or maize DNA, suggesting the notion that a large fraction of the rice genome (approximately 50%) is single copy.