Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author's Perspective.
Dhurat R,M. S. Sukesh +1 more
TLDR
The principles and preparation methods of PRP are reviewed based on available literature and a perspective in standardizing a safe, simple protocol that can be followed to obtain an optimal consistent platelet yield is placed.Abstract:
The utility of platelet-rich plasma (PRP) has spanned various fields of dermatology from chronic ulcer management to trichology and aesthetics, due to its role in wound healing. Though PRP is being used over a long time, there is still confusion over proper terminology to define, classify and describe the different variations of platelet concentrates. There is also a wide variation in the reported protocols for standardization and preparation of PRP, in addition to lack of accurate characterization of the tested products in most articles on the topic. Additionally, the high cost of commercially available PRP kits, precludes its use over a larger population. In this article, we review the principles and preparation methods of PRP based on available literature and place our perspective in standardizing a safe, simple protocol that can be followed to obtain an optimal consistent platelet yield.read more
Citations
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Guided bone regeneration: materials and biological mechanisms revisited.
TL;DR: Experimental data suggest that different modifications of the physicochemical and mechanical properties of membranes may promote bone regeneration, Nevertheless, the precise role of membrane porosities for the barrier function of GBR membranes still awaits elucidation.
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A Review of Platelet-Rich Plasma: History, Biology, Mechanism of Action, and Classification.
Rubina Alves,Ramon Grimalt +1 more
TL;DR: The knowledge of the biology, mechanism of action, and classification of the PRP should help clinicians better understand this new therapy and to easily sort and interpret the data available in the literature regarding PRP.
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Platelets in wound healing and regenerative medicine.
TL;DR: The biological mechanisms underlying PRP’s regenerative effects, the different methods of preparation and application of these biomaterials, and the controversies and future prospects related to the use of PRP in regenerative medicine are described.
Journal ArticleDOI
Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt pathway, Growth-Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development.
Pietro Gentile,Simone Garcovich +1 more
TL;DR: The significant improvements in intraoperative stem cell approaches, from in vivo models to clinical investigations, are reviewed and the potential regenerative instruments and functions of various cell populaces in the hair regrowth process are discussed.
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Concentrations of Blood Components in Commercial Platelet-Rich Plasma Separation Systems: A Review of the Literature.
TL;DR: There is a large heterogeneity between PRP separation systems regarding concentrations of platelets, leukocytes, and growth factors in PRP and the choice for the most appropriate type of PRP should be based on the specific clinical field of application.
References
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Platelet-rich plasma: evidence to support its use
TL;DR: Not all currently marketed PRP devices are qual; some do not concentrate viably active platelets n sufficient numbers to produce a healing enhance- herefore, the term PRP is preferred to autologous latelet gel, plasma-rich growth factors (PRGFs), or a ere autOLOGous platelet concentrate.
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Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF).
TL;DR: This classification of the different platelet concentrates into four categories, depending on their leucocyte and fibrin content, should help to elucidate successes and failures that have occurred so far, as well as providing an objective approach for the further development of these techniques.
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Platelet-rich plasma (PRP): what is PRP and what is not PRP?
TL;DR: The definition of PRP, its safety, its proper development, and its most efficacious means of application are discussed.
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Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration.
TL;DR: The Platelet concentration required for a positive PRP effect on bone regeneration seems to span a very limited range, and advantageous biological effects seem to occur when PRP with a platelet concentration of approximately 1,000,000/microl is used.
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Platelet α-granules
Paul Harrison,E. Martin Cramer +1 more
TL;DR: The α-granule is a unique secretory organelle in that it exhibits further compartmentalization and acquires its protein content via two distinct mechanisms: (1) biosynthesis predominantly at the megakaryocyte (MK) level (with some vestigial platelet synthesis) and (2) endocytosis and pinocythosis at both the MK and circulating platelet levels (e.g. platelet factor 4) as discussed by the authors.