Journal ArticleDOI
Problems with DNA barcodes for species delimitation: ‘Ten species’ of Astraptes fulgerator reassessed (Lepidoptera: Hesperiidae)
TLDR
At least three, but not more than seven mtDNA clades that may correspond to cryptic species are supported by the evidence.Abstract:
Hebert and colleagues (2004) used a short region of the mitochondrial Cytochrome oxidase subunit I gene as a delimiter for ten putative species from among 466 individuals of the skipper butterfly currently known as Astraptes fulgerator from Guanacaste, Costa Rica. Their data are reanalysed to assess cluster stability and clade support using Neighbor‐Joining bootstrap, population aggregation analysis and cladistic haplotype analysis. At least three, but not more than seven mtDNA clades that may correspond to cryptic species are supported by the evidence. Additional difficulties with Hebert et al.’s interpretation of the data are discussed.read more
Citations
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Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified
TL;DR: It is demonstrated that the presence of COI numts makes this goal difficult to achieve when numts are prevalent and can introduce serious ambiguity into DNA barcoding, which strives for rapid and inexpensive generation of molecular species tags.
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Does the DNA barcoding gap exist? - a case study in blue butterflies (Lepidoptera: Lycaenidae).
Martin Wiemers,Konrad Fiedler +1 more
TL;DR: An analysis of intra- and interspecific variation in the butterfly family Lycaenidae which includes a well-sampled clade with a peculiar characteristic that facilitates the recognition of species as reproductively isolated units even in allopatric populations indicates that the "barcoding gap" is an artifact of insufficient sampling across taxa.
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The seven deadly sins of DNA barcoding
TL;DR: An assessment of seven deficiencies that are common in the DNA barcoding literature are provided, and some potential improvements for its adaptation and adoption towards more reliable and accurate outcomes are outlined.
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A Genomic Perspective on the Shortcomings of Mitochondrial DNA for “Barcoding” Identification
TL;DR: The natural history of mtDNA is reviewed and problems for barcoding which are particularly associated with mtDNA and inheritance are discussed, including reduced effective population size, maternal inheritance, recombination, inconsistent mutation rate, heteroplasmy, and compounding evolutionary processes.
Journal ArticleDOI
Species delimitation: new approaches for discovering diversity.
TL;DR: An increased interest in species delimitation has arisen because of growing concern over threats to biodiversity and the desire to describe as many species as possible as quickly and accurately as possible before they disappear.
References
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Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
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Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
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Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species
TL;DR: It is indicated that sequence divergences at COI regularly enable the discrimination of closely allied species in all animal phyla except the Cnidaria and constraints on intraspecific mitochondrial DNA divergence arising through selective sweeps mediated via interactions with the nuclear genome.