Journal ArticleDOI
Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O.
TLDR
A fast and simple method to quantitate the extent of adipose conversion by staining the accumulated lipid with Oil red O and determining the amount of extracted dye at 510 nm is reported.Abstract:
Cultured 3T3-F442A cells differentiate into adipocytes and accumulate lipid droplets in the cytoplasm. When fat cells are stained with Oil red O, the degree of staining seems to be proportional to the extent of cell differentiation. We report here a fast and simple method to quantitate the extent of adipose conversion by staining the accumulated lipid with Oil red O and determining the amount of extracted dye at 510 nm. The results show that Oil red O specifically stains triglycerides and cholesteryl oleate but no other lipids. This technique is a valuable tool for processing large numbers of cell cultures or samples in which adipose differentiation and/or accumulated triglycerides is to be quantitated.read more
Citations
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Journal ArticleDOI
Expression of a Constitutively Active Akt Ser/Thr Kinase in 3T3-L1 Adipocytes Stimulates Glucose Uptake and Glucose Transporter 4 Translocation
TL;DR: It is shown that Akt can regulate glucose uptake and metabolism and is expressed in 3T3-L1 adipocytes directed lipid but not glycogen synthesis.
Journal ArticleDOI
Identification of Telmisartan as a Unique Angiotensin II Receptor Antagonist With Selective PPARγ–Modulating Activity
Stephen C. Benson,Harrihar A. Pershadsingh,Christopher I. Ho,Amar G. Chittiboyina,Prashant V. Desai,Michal Pravenec,N. Qi,Jiaming Wang,Mitchell A. Avery,Theodore W. Kurtz +9 more
TL;DR: In this paper, the authors showed that telmisartan, a structurally unique angiotensin II receptor antagonist used for the treatment of hypertension, can function as a partial agonist of peroxisome proliferator-activated receptor-gamma (PPARgamma) and reduce glucose, insulin, and triglyceride levels in rats fed a high-fat, high-carbohydrate diet.
Journal ArticleDOI
Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality
Ichiro Sekiya,Benjamin L. Larson,Jason Smith,Radhika Pochampally,Jian-Guo Cui,Darwin J. Prockop +5 more
TL;DR: Assays for adipogenesis demonstrated that the adipogenic potential of cultures was directly related to their ability to generate single‐cell‐derived colonies and their enrichment for RS‐1A cells, and cultures enriched for RS-1B cells showed the greatest potential to differentiate into cartilage in a serum‐free system.
Journal ArticleDOI
Effect of genetic variation in the organic cation transporter 1 (OCT1) on metformin action.
Yan Shu,Steven A. Sheardown,Chaline Brown,Ryan P. Owen,Shuzhong Zhang,Richard A. Castro,Alexandra G. Ianculescu,Lin Yue,Joan C. Lo,Esteban G. Burchard,Claire M. Brett,Kathleen M. Giacomini +11 more
TL;DR: The data indicate that OCT1 is important for metformin therapeutic action and that genetic variation in OCT1 may contribute to variation in response to the drug.
Journal ArticleDOI
Regulation, function, and dysregulation of endocannabinoids in models of adipose and beta-pancreatic cells and in obesity and hyperglycemia.
Isabel Matias,Marie-Paule Gonthier,Pierangelo Orlando,Vassilis Martiadis,Luciano De Petrocellis,Cristina Cervino,Stefania Petrosino,Laurence Hoareau,Franck Festy,Renato Pasquali,Régis Roche,Mario Maj,Uberto Pagotto,Palmiero Monteleone,Vincenzo Di Marzo +14 more
TL;DR: Peripheral endocannabinoid overactivity might explain why CB(1) blockers cause weight-loss independent reduction of lipogenesis, of hypoadiponectinemia, and of hyperinsulinemia in obese animals and humans.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Histochemistry, theoretical and applied
TL;DR: The new Pearse bids fair to become the leader, even amongst so notable a collection of books devoted entirely or largely to histochemical techniques.
Book
Animal Tissue Techniques
TL;DR: Animal tissue techniques, Animal tissue techniques , مرکز فناوری اطلاعات و اصاع رسانی, کδاوρزی
Journal ArticleDOI
An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion
Howard Green,Olaniyi Kehinde +1 more
TL;DR: Lipogenic and lipolytic hormones and drugs affect the rate of synthesis and accumulation of triglyceride by 3T3-L1 cells, but in contrast to bromodeoxyuridine, these modulating agents do not seem to affect the proportion of cells which undergoes the adipose conversion.
Journal ArticleDOI
An established pre-adipose cell line and its differentiation in culture
Howard Green,Mark Meuth +1 more
TL;DR: The conversion of 3T3-L1 from pre-adipose to adipose cells therefore involves a process of differentiation which can be studied under cell culture conditions.