Journal ArticleDOI
Tumor measurement in the nude mouse.
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TLDR
In this study, the pitfalls of tumor measurement in the nude mouse were evaluated and recommendations are made for future work employing tumor measurement.Abstract:
In this study, the pitfalls of tumor measurement in the nude mouse were evaluated. Regarding intermethod variation, diameters of subcutaneous tumors in nude mice were expressed as length, area, and volume; tumor weights were also recorded. These measurements were all compared to a reference standard: water displacement volume. Estimates of area and volume derived from caliper measurements correlated well with water displacement volume (r = 0.97 and 0.98, respectively). At necropsy, tumor weight was the most consistent and reproducible reflection of tumor volume (r = 1.0000). Regarding interobserver variation, mean absolute difference among volumes determined by several investigators who measured the same tumors in living animals was determined. This averaged 15% of the mean calculated volume. Regarding intraobserver variation, observers measured four separate masses in nude mice eight times each. The observers were prevented from realizing that the same animals were being repeatedly evaluated. Volumes were compared in order to quantify the average variation that occurs when a single investigator repeatedly measures the same mass. When large masses were measured, this error was 7%; when small masses were measured, the error was 27%. Recommendations are made for future work employing tumor measurement.read more
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Determination of subcutaneous tumor size in athymic (nude) mice
TL;DR: In this article, the authors compared the use of 19 different formulas for determining the size of subcutaneous tumors grown as xenografts in nude mice (2 for determining tumor area, 3 for tumor diameter, and 14 for calculating tumor volume).
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Cloning and characterization of FGF23 as a causative factor of tumor-induced osteomalacia
Takashi Shimada,Satoru Mizutani,Takanori Muto,Takashi Yoneya,Rieko Hino,Shu Takeda,Yasuhiro Takeuchi,Toshiro Fujita,Seiji Fukumoto,Takeyoshi Yamashita +9 more
TL;DR: It is concluded that overproduction of F GF23 causes TIO, whereas mutations in the FGF23 gene result in autosomal dominant hypophosphatemic rickets possibly by preventing proteolytic cleavage and enhancing biological activity of FGF 23.
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Pharmacological Rescue of Mutant p53 Conformation and Function
TL;DR: Small synthetic molecules identified promoted the stability of wild-type p53 but also allowed mutant p53 to maintain an active conformation, enabling it to activate transcription and to slow tumor growth in mice.
Journal ArticleDOI
Cancer cell–selective in vivo near infrared photoimmunotherapy targeting specific membrane molecules
Makoto Mitsunaga,Mikako Ogawa,Nobuyuki Kosaka,Lauren T Rosenblum,Peter L. Choyke,Hisataka Kobayashi +5 more
TL;DR: A new type of molecular-targeted cancer therapy, photoimmunotherapy (PIT), that uses a target-specific photosensitizer based on a near-infrared (NIR) phthalocyanine dye, IR700, conjugated to monoclonal antibodies (mAbs) targeting epidermal growth factor receptors is developed.
References
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Journal Article
Immunology of Spontaneous Mammary Carcinomas in Mice V. Acquired Tumor Resistance and Enhancement in Strain A Mice Infected with Mammary Tumor Virus
Mohammed A. M. Attia,David Weiss +1 more
TL;DR: Immunization of Strain A/Crgl female mice with living tissue and crude cell membrane preparations of recently arisen isogenic spontaneous mammary carcinomas conferred a marked degree of heightened reactivity against a subsequent challenge with the tumors.
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The effect of measuring error on the results of therapeutic trials in advanced cancer
TL;DR: In this study, 16 experienced oncologists each measured 12 simulated tumor masses employing their usual clinical methods but two pairs of these tumors were identical in size, which permitted a total of 64 measurement comparisons of the same investigator measuring the same size mass and 1920 comparisons of different investigators measuring thesame size mass.
Journal ArticleDOI
Growth and chemotherapeutic response in athymic mice of tumors arising from human glioma-derived cell lines.
TL;DR: This model allows cell lines derived from human gliomas to be grown in animal hosts, thereby providing a potential means for evaluating growth parameters and chemotherapeutic responsiveness of tumors derived from individual humangliomas or cell lines.
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Gastrin-Enhanced Tumor Growth of a Xenotransplantable Human Gastric Carcinoma in Nude Mice
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