Vero response to a cytotoxin of Escherichia coli.
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A cytotoxin was found in culture filtrates of a number of Escherichia coli strains that differed from the known heat-stable and heat-labile enterotoxins of E. coli.Abstract:
A cytotoxin was found in culture filtrates of a number of Escherichia coli strains that differed from the known heat-stable and heat-labile enterotoxins of E. coli. It was cytotoxic for Vero but not for Y-1 or CHO cells, and its effect on Vero was distinctly different from that of heat-labile enterotoxin. It was labile to heat and antigenically different from heat-labile enterotoxin, and membrane filtration indicated a molecular weight of 10,000 to 30,000.read more
Citations
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Diarrheagenic Escherichia coli
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TL;DR: The current level of understanding of the pathogenesis of the diarrheagenic E. coli strains is discussed and how their pathogenic schemes underlie the clinical manifestations, diagnostic approach, and epidemiologic investigation of these important pathogens are described.
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Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections
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Infection by verocytotoxin-producing Escherichia coli.
TL;DR: The best strategies for diagnosing human VTEC infection including testing for the presence of free VT in fecal filtrates and examining fecal cultures for VTEC by means of deoxyribonucleic acid probes that specify genes encoding VT1 and VT2 are currently confined to specialized laboratories and await commercial development for wider use.
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References
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Journal ArticleDOI
Test for Escherichia coli Enterotoxin Using Infant Mice: Application in a Study of Diarrhea in Children in Honolulu
TL;DR: Use of the infant-mouse test in a study of 37 children with diarrhea in Honolulu revealed no enterotoxin-producing coliform bacteria in the stools, in contrast to studies reported from India, where such strains were found in a large proportion of undifferentiated cases of acute diarrhea in adults.
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An outbreak of enteropathogenic escherichia coli foodborne disease traced to imported french cheese
TL;DR: This is believed to be the first adequately documented occurrence of enteropathogenic E. coli foodborne disease in the U.S.A. traced to imported French cheese.
Journal ArticleDOI
The titration of cholera toxin and antitoxin in the rabbit ileal loop.
George J. Kasai,William Burrows +1 more
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Assay of Escherichia coli heat-labile enterotoxin with vero cells
TL;DR: The continuous cell line of African green monkey kidney, Vero, showed characteristic morphological changes in response to culture filtrates from toxigenic strains of Escherichia coli and was the simplest and most economical to maintain in the laboratory.
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Classification of Enterotoxins on the Basis of Activity in Cell Culture
Gerald T. Keusch,Sam T. Donta +1 more
TL;DR: These cell culture systems may serve as convenient models for the study of the mechanism of action of both classes of enterotoxin, and raise the possibility that some enterotoxins may not interact with the adenyl cyclase-cyclic AMP system.