Journal ArticleDOI
Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining
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TLDR
The available cytochemical data suggest that the Ag-AS reaction stains chromosomal proteins at the NOR rather than the rDNA itself, and this was critically demonstrated by Ag- AS staining of the nucleolus organizer regions in karyotypes of the same species and cell lines used for locating the ribosomal cistrons by DNA/RNA in situ hybridization.Abstract:
A simple ammoniacal silver staining procedure, designated Ag-AS, differentially stains the chromosomal locations of ribosomal DNA in certain mammalian species. This was critically demonstrated by Ag-AS staining of the nucleolus organizer regions in karyotypes of the same species and cell lines used for locating the ribosomal cistrons by DNA/RNA in situ hybridization. With Ag-AS, silver stained NORs (Ag-NORs) are visualized as black spherical bodies on yellow-brown chromosome arms. Ag-NORs were visualized throughout mitosis at the secondary constrictions in the rat kangaroo, Seba's fruit bat, Indian muntjac, and Rhesus monkey. The Chinese hamster and cattle have telomeric Ag-NORs, the mouse subcentromeric Ag-NORs, and the field vole Ag-NORs as minute short arms or choromosomal satellites. Ag-NORs occur at both secondary constrictions and at telomeres in the cotton rat. Variability in Ag-NOR pattern included differences in the number of Ag-NORs per cell within a cell population, size of Ag-NORs among chromosomes of a complement, and presence of Ag-NOR on particular chromosomes in two cell lines of the Chinese hamster. The available cytochemical data suggest that the Ag-AS reaction stains chromosomal proteins at the NOR rather than the rDNA itself.read more
Citations
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Journal ArticleDOI
Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method.
W. M. Howell,Deborah Black +1 more
TL;DR: A 1-step silver-staining technique, requiring only 2 min to perform, is described for the differential staining of nucleolus organizer regions.
Journal ArticleDOI
Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination
TL;DR: The meiotic and mitotic defects of rad50 mutants can be accounted for economically by the proposal that meiotic recombination, meiotic chromosome pairing, and vegetative DNA repair all use a common chromosomal homology search that involves RAD50 function.
Journal ArticleDOI
Meiotic Pachytene Arrest in MLH1-Deficient Mice
Winfried Edelmann,Paula E. Cohen,Michael F. Kane,Kirkland Lau,Bernice E. Morrow,Samuel E. Bennett,Asad Umar,Thomas A. Kunkel,Giorgio Cattoretti,Raju S.K. Chaganti,Jeffrey W. Pollard,Richard D. Kolodner,Raju Kucherlapati +12 more
TL;DR: The different phenotypes of the three types of mutant mice suggest that these three genes may have independent functions in mammalian meiosis.
Journal ArticleDOI
An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes
TL;DR: The silver staining procedure for NOR's was simplified and standardized through control of the chemical and physical conditions during silver impregnation and developing.
Journal ArticleDOI
Developing luminescent silver nanodots for biological applications
TL;DR: This critical review of studies on silver nanodots in inert environments and in aqueous solutions is revisited with an effort to decipher the relations between their chemical/photophysical properties and their structures.
References
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Journal ArticleDOI
Location of ribosomal DNA in the human chromosome complement.
TL;DR: Hybridization of (3)H-labeled ribosomal RNA to human chromosomes on slides resulted in specific labeling of the satellite regions of chromosomes 13, 14, 15, 21, and 22, with an over-all efficiency of about 5%.
Journal ArticleDOI
Differential staining of the satellite regions of human acrocentric chromosomes
TL;DR: Es wird eine neue Ammoniak-Silber-Methode für das Färben menschlicher Chromosomen in der Metaphase beschrieben, wodurch die Satelliten aller 10 akrozentrischen chromosomen zu unterscheiden sind.
Journal ArticleDOI
Location of the genes coding for 18S and 28S ribosomal RNA in the human genome
TL;DR: It was concluded that the probability of a chromosome being involved in the formation of a common nucleolus is not a simple function of its rDNA content and other possible factors are considered.
Journal ArticleDOI
Distribution of 18+28S ribosomal genes in mammalian genomes.
TL;DR: In situ hybridization with 3H 18S and 28S ribosomal RNA from Xenopus laevis has been used to study the distribution of DNA sequences coding for these RNAs (the nucleolus organizing regions) in the genomes of six mammals.
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