E
Eva C. Thuenemann
Researcher at Norwich Research Park
Publications - 23
Citations - 2133
Eva C. Thuenemann is an academic researcher from Norwich Research Park. The author has contributed to research in topics: Tobacco mosaic virus & RNA. The author has an hindex of 12, co-authored 19 publications receiving 1825 citations. Previous affiliations of Eva C. Thuenemann include Norwich University & Sainsbury Laboratory.
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Journal ArticleDOI
pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants
TL;DR: V vectors have been developed which allow the direct cloning of genes into the binary plasmid by both restriction enzyme-based cloning and GATEWAY recombination and N- or C-terminal histidine tags may be fused to the target sequence as required.
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miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
Attila Molnar,Frank Schwach,Frank Schwach,David J. Studholme,Eva C. Thuenemann,David C. Baulcombe +5 more
TL;DR: The unicellular alga Chlamydomonas reinhardtii is shown to contain miRNAs, putative evolutionary precursors of mi RNAs and species of siRNAs resembling those in higher plants, indicating that complex RNA-silencing systems evolved before multicellularity and were a feature of primitive eukaryotic cells.
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Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii
Attila Molnar,Andrew R. Bassett,Eva C. Thuenemann,Frank Schwach,Shantanu Karkare,Stephan Ossowski,Detlef Weigel,David C. Baulcombe +7 more
TL;DR: A novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae is developed.
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A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles.
TL;DR: The results show that transient expression can be used to produce immunologically relevant complex heteromultimeric structures in plants in a matter of days and have implications beyond the realm of veterinary vaccines.
Journal ArticleDOI
Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity to accommodate foreign proteins.
Hadrien Peyret,Annick Gehin,Eva C. Thuenemann,Donatienne Blond,Aadil El Turabi,Lucy Beales,Dean Clarke,Robert J.C. Gilbert,Elizabeth E. Fry,David I. Stuart,Kris Holmes,Nicola J. Stonehouse,M Whelan,William Rosenberg,George P. Lomonossoff,David J. Rowlands +15 more
TL;DR: T tandem core technology paves the way for the display of natively folded proteins on the surface of HBc particles either through direct fusion or through non-covalent attachment via a nanobody.