F
Frank Sainsbury
Researcher at Commonwealth Scientific and Industrial Research Organisation
Publications - 82
Citations - 3504
Frank Sainsbury is an academic researcher from Commonwealth Scientific and Industrial Research Organisation. The author has contributed to research in topics: Cowpea mosaic virus & Protease. The author has an hindex of 27, co-authored 72 publications receiving 2927 citations. Previous affiliations of Frank Sainsbury include University of Sydney & University of Queensland.
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Journal ArticleDOI
pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants
TL;DR: V vectors have been developed which allow the direct cloning of genes into the binary plasmid by both restriction enzyme-based cloning and GATEWAY recombination and N- or C-terminal histidine tags may be fused to the target sequence as required.
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Extremely High-Level and Rapid Transient Protein Production in Plants without the Use of Viral Replication
TL;DR: A system based on a disabled version of cowpea mosaic virus RNA-2, which overcomes limitations on insert size and introduces biocontainment, provides an ideal vehicle for high-level expression that does not rely on viral replication of transcripts.
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Bioengineering virus‐like particles as vaccines
Linda H.L. Lua,Natalie K. Connors,Frank Sainsbury,Yap P. Chuan,Nani Wibowo,Anton P. J. Middelberg +5 more
TL;DR: This review highlights historical and emerging VLP vaccine approaches that seek to specifically engineer a desirable immune response through modular VLP design, and those that seeks to improve bioprocess efficiency through inhibition of intracellular assembly to allow optimal use of existing purification technologies prior to cell‐free VLP assembly.
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A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats.
Sam T. Mugford,Xiaoquan Qi,Saleha Bakht,Lionel Hill,Eva Wegel,Richard K. Hughes,Kalliopi Papadopoulou,Rachel E. Melton,Mark Philo,Frank Sainsbury,George P. Lomonossoff,Abhijeet Deb Roy,Rebecca J. M. Goss,Anne Osbourn +13 more
TL;DR: The cloning of Saponin-deficient 7 (Sad7) is reported and it is shown that this gene encodes a functional SCPL acyltransferase, SCPL1, that is able to catalyze the synthesis of both N-methyl anthraniloyl - and benzoyl-derivatized forms of avenacin.
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Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants
Katrin Geisler,Richard K. Hughes,Frank Sainsbury,George P. Lomonossoff,Martin Rejzek,Shirley A. Fairhurst,Carl-Erik Olsen,Mohammed Saddik Motawia,Rachel E. Melton,Andrew M. Hemmings,Søren Bak,Anne Osbourn +11 more
TL;DR: It is shown that the oat AsCYP51H10 enzyme has been recruited from primary metabolism and has acquired a different function compared to other characterized members of the plant CYP51 family—as a multifunctional stereo- and regio-specific hydroxylase in plant specialized metabolism.