Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration

Summary
• Over the last decade, technological developments have made it possible to quickly and nondestructively assess, in situ, the chlorophyll (Chl) status of plants. We evaluated the performance of these optical methods, which are based on the absorbance or reflectance of certain wavelengths of light by intact leaves.
• As our benchmark, we used standard extraction techniques to measure Chla, Chlb, and total Chl content of paper birch (Betula papyrifera) leaves. These values were compared with the nominal Chl index values obtained with two hand-held Chl absorbance meters and several reflectance indices correlated with foliar Chl.
• The noninvasive optical methods all provided reliable estimates of relative leaf Chl. However, across the range of Chl contents studied (0.0004–0.0455 mg cm−2), some reflectance indices consistently out-performed the hand-held meters. Most importantly, the reflectance indices that performed best 

were not those most commonly used in the literature.
• We report equations to convert from index values to actual Chl content, but caution that differences in leaf structure may necessitate species-specific calibration equations.

https://nph.onlinelibrary.wiley.com/doi/epdf/10.1046/j.0028-646X.2001.00289.x

An evaluation of noninvasive methods to estimate foliar chlorophyll content

A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants

Kluyvera ascorbata SUD165 and a siderophore-overproducing mutant of this bacterium, K. ascorbata SUD165/26, were used to inoculate tomato, canola, and Indian mustard seeds which were then grown in ...

Plant growth-promoting bacteria that decrease heavy metal toxicity in plants

Evaluating physiological responses of plants to salinity stress

A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method. Unlike the acetone method where grinding and subsequent centrifugation are essential, this method makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide. The new method was found to be as efficient as acetone for chlorophyll extraction and superior in terms of chlorophyll stability.

G. F. Israelstam

Papers

A method for the extraction of chlorophyll from leaf tissue without maceration