Journal ArticleDOI
A method for the extraction of chlorophyll from leaf tissue without maceration
J. D. Hiscox,G. F. Israelstam +1 more
TLDR
A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method, which makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide.Abstract:
A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method. Unlike the acetone method where grinding and subsequent centrifugation are essential, this method makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide. The new method was found to be as efficient as acetone for chlorophyll extraction and superior in terms of chlorophyll stability.read more
Citations
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Journal ArticleDOI
Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration
TL;DR: Results show that salinity tolerance of Kharchia 65 as manifested by lower decrease in biomass and grain yield is associated with higher antioxidant activity, osmolyte concentration and potassium contents, and lower H2O2, TBARS and sodium contents than KRL 19.
Journal ArticleDOI
An evaluation of noninvasive methods to estimate foliar chlorophyll content
TL;DR: This article evaluated the performance of these optical methods, which are based on the absorbance or reflectance of certain wavelengths of light by intact leaves, and found that the reflectance indices that performed best were not those most commonly used in the literature.
Journal ArticleDOI
A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants
TL;DR: In this article, the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants was reevaluated, and the problem of degradation resulting from the presence of acidic lichen substances was specifically addressed.
Journal ArticleDOI
Plant growth-promoting bacteria that decrease heavy metal toxicity in plants
TL;DR: Two bacterial strains were used to inoculate tomato, canola, and Indian mustard seeds which were then grown in soil in the presence of either nickel, lead, or zinc, and both were effective at relieving a portion of the growth inhibition caused by the metals.
Journal ArticleDOI
Evaluating physiological responses of plants to salinity stress
TL;DR: This paper discusses how to quantify the impact of salinity on different traits, and illustrates how indices can be used to identify relationships amongst the proposed traits to identify which traits are the most important contributors to salinity tolerance.
References
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Journal ArticleDOI
THE QUANTITATIVE ANALYSIS OF CHLOROPHYLLS a AND b IN PLANT EXTRACTS
TL;DR: A detailed survey of the complications encountered in the quantitative determination of chlorophyll can be found in this article, with a detailed description of one of the most commonly used methods for the extraction and determination of these pigments.
Journal ArticleDOI
Improved extraction of chlorophyll a and b from algae using dimethyl sulfoxide
W. Thomas Shoaf,Bruce W. Lium +1 more
TL;DR: Dimethyl sulfoxide (DMSO) and 90% acetone extracted equal amounts of chlorophyll from diatoms and blue-green algae, but DMSO was superior to 90%acetone for all green algae tested giving 2-60 times more chlorophyLL depending on the species as discussed by the authors.
Journal ArticleDOI
A new tissue essential to necrophylactic periderm formation in the bark of four conifers
TL;DR: It is concluded that NIT precedes formation of necrophylactic periderms (NP), may provide environment necessary for NP formation in tissues internally abutting NIT, and is a marker for distinguishing NP from exophylacticPeriderms.
Journal ArticleDOI
New concepts and terminology of coniferous periderms: necrophylactic and exophylactic periderms
D. B. Mullick,G. D. Jensen +1 more
TL;DR: Field observations were made on wound and pathological periderms, regardless of the causal agent, and periderm formed at abscission zones, old resin blisters and rhytidomes in Abies amabilis.
Journal ArticleDOI
The role of wound periderm in the resistance of eastern larch and jack pine to dwarf mistletoe
F. H. Tainter,D. W. French +1 more
TL;DR: Wound periderm appears to be a common form of resistance reaction in the Arceuthobium pusillum – Larix laricina combination, which may account for the relative scarcity of aerial shoots on this host and restricted development of the endophytic system.