J. E. Scott

TL;DR: Model experiments with pure polyanions, or artifically carboxylated, phosphorylated and sulphated liver sections, showed that binding of dye to carboxyate or phosphate groups ceased at low electrolyte concentrations, whereas dye continued to be held by sulphate ester groups at concentrations five to ten times as high.

TL;DR: The relevance of Alcian blue aggregation to the speed of staining, its use in critical electrolyte concentration techniques, and in microspectrophotometry is considered.

TL;DR: This, the first chemical method for localising acid mucopolysaccharides, is compared with “critical electrolyte concentration” techniques using Alcian blue for the same purposes and preliminary data presented here show that both sets of results are compatible.

TL;DR: Comparison of oxidation rates of polyanions are better carried out in the presence of added salt, to reduce the non-specific effects of the electrostatic fields.

TL;DR: The combination of pyronin, methyl green, malachite green, crystal violet, and Alcian Blue with a large number of polynucleotides and acidic polysaccharides has been investigated and the “critical electrolyte concentration” approach has been used to provide a measure of affinity between dye and substrate.