Institution
Canadian Red Cross
Nonprofit•Ottawa, Ontario, Canada•
About: Canadian Red Cross is a nonprofit organization based out in Ottawa, Ontario, Canada. It is known for research contribution in the topics: Antibody & Antigen. The organization has 948 authors who have published 780 publications receiving 26733 citations. The organization is also known as: CRC & Canadian Red Cross Society.
Topics: Antibody, Antigen, Thrombin, Antithrombin, Population
Papers published on a yearly basis
Papers
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TL;DR: The generation of mice deficient in Flk-1 by disruption of the gene using homologous recombination in embryonic stem (ES) cells is reported, indicating that FlK-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo.
Abstract: The receptor tyrosine kinase Flk-1 (ref. 1) is believed to play a pivotal role in endothelial development. Expression of the Flk-1 receptor is restricted to endothelial cells and their embryonic precursors, and is complementary to that of its ligand, vascular endothelial growth factor (VEGF), which is an endothelial-specific mitogen. Highest levels of flk-1 expression are observed during embryonic vasculogenesis and angiogenesis, and during pathological processes associated with neovascularization, such as tumour angiogenesis. Because flk-1 expression can be detected in presumptive mesodermal yolk-sac blood-island progenitors as early as 7.0 days postcoitum, Flk-1 may mark the putative common embryonic endothelial and haematopoietic precursor, the haemangioblast, and thus may also be involved in early haematopoiesis. Here we report the generation of mice deficient in Flk-1 by disruption of the gene using homologous recombination in embryonic stem (ES) cells. Embryos homozygous for this mutation die in utero between 8.5 and 9.5 days post-coitum, as a result of an early defect in the development of haematopoietic and endothelial cells. Yolk-sac blood islands were absent at 7.5 days, organized blood vessels could not be observed in the embryo or yolk sac at any stage, and haematopoietic progenitors were severely reduced. These results indicate that Flk-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo.
4,063 citations
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TL;DR: Model experiments with pure polyanions, or artifically carboxylated, phosphorylated and sulphated liver sections, showed that binding of dye to carboxyate or phosphate groups ceased at low electrolyte concentrations, whereas dye continued to be held by sulphate ester groups at concentrations five to ten times as high.
Abstract: The application of the “critical electrolyte concentration” (CEC) concept to the differentiation of acidic glycosaminglycans (mucopolysaccharides) is described. Alcian Blue 8GX stains with increasing selectivity as increasing amounts of magnesium chloride are incorporated into the dye solution. Model experiments with pure polyanions, or artifically carboxylated, phosphorylated and sulphated liver sections, showed that binding of dye to carboxylate or phosphate groups ceased at low electrolyte concentrations (< 0.3M) whereas dye continued to be held by sulphate ester groups at concentrations five to ten times as high. The similarity to the well established cetylpyridinium system for polyanion fractionation is discussed. Sections of tissues chosen to contain predominantly or characteristically carboxylated mucins, and/or sulphate ester polyanions showed a staining pattern entirely similar to the model sections. Goblet cell mucin in rat ileum stained at < 0.4M MgCl2, Cartilage at < 0.6M MgCl2, mast cells at < 0.75M, and corneal stroma at < 1.0M. These results are in agreement with the known contents of sialo-mucin, chondroitin sulphate, heparin and keratansulphate, respectively. The conditions in which this principle can be used in a practical technique are described.
982 citations
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TL;DR: A physiological role of TNF-alpha and IFN-gamma expression in the placental villi may be to regulate the apoptotic death of villous cytotrophoblasts to predict potential harmful effects on placental development and function following aberrant inflammatory cytokine expression triggered by intravillous infections.
371 citations
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TL;DR: It was found that transfusion reactions were much more frequently associated with platelet transfusion than with red cell transfusion, and the significant relationship between reaction and the increasing age of the component suggests that cytokines released in the component during storage may be responsible for many reactions to blood components.
332 citations
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TL;DR: The clinical observations suggest that peripheral-blood and lymph-node lymphocytes shown to have immunoglobulins belong to the bone-marrow-derived " B "-cell population, and patients with active rheumatoid arthritis show an increased proportion in their peripheral blood.
298 citations
Authors
Showing all 948 results
Name | H-index | Papers | Citations |
---|---|---|---|
Jack Hirsh | 146 | 734 | 86332 |
Jeffrey S. Ginsberg | 101 | 343 | 37014 |
Robin S. Roberts | 98 | 288 | 38486 |
Theodore E. Warkentin | 96 | 440 | 34706 |
John G. Kelton | 83 | 326 | 29537 |
Sam Schulman | 74 | 460 | 35175 |
Morris A. Blajchman | 71 | 329 | 22824 |
Richard A. Rachubinski | 61 | 173 | 10915 |
Nancy M. Heddle | 57 | 255 | 10742 |
Victor S. Blanchette | 55 | 289 | 15148 |
Shida Yousefi | 52 | 143 | 9975 |
Haresh Kirpalani | 52 | 226 | 10229 |
Barbara Schmidt | 50 | 170 | 10140 |
James W. Smith | 50 | 194 | 7791 |
Harvey G. Klein | 49 | 247 | 11097 |