K
Karen Bolitho
Researcher at Plant & Food Research
Publications - 8
Citations - 2730
Karen Bolitho is an academic researcher from Plant & Food Research. The author has contributed to research in topics: Ripening & Genetically modified tomato. The author has an hindex of 7, co-authored 8 publications receiving 2227 citations. Previous affiliations of Karen Bolitho include HortResearch.
Papers
More filters
Journal ArticleDOI
Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants
Roger P. Hellens,Andrew C. Allan,Ellen N. Friel,Karen Bolitho,Karryn Grafton,Matthew D. Templeton,Sakuntala Karunairetnam,Andrew P. Gleave,William A. Laing +8 more
TL;DR: In this article, a series of plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves, are described and compared to conventional binary vectors for stable transformation such as transformation selection genes.
Journal ArticleDOI
An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae
Kui Lin-Wang,Karen Bolitho,Karryn Grafton,A.J. Kortstee,Sakuntala Karunairetnam,Tony K. McGhie,Richard V. Espley,Roger P. Hellens,Andrew C. Allan +8 more
TL;DR: This family-wide study of rosaceous R2R3 MYBs provides insight into the evolution of this plant trait, which has implications for the development of new coloured fruit and flowers, as well as aiding the understanding of temporal-spatial colour change.
Journal Article
An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae
Kui Lin-Wang,Karen Bolitho,Karryn Grafton,A.J. Kortstee,Sakuntala Karunairetnam,Tony K. McGhie,Richard V. Espley,Roger P. Hellens,Andrew C. Allan +8 more
TL;DR: In this paper, the authors used gene specific primers to show that the three activators of apple anthocyanin (myb10/myb1/myBA) are likely alleles of each other.
Journal ArticleDOI
A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway
Robert J. Schaffer,Ellen N. Friel,Edwige J. F. Souleyre,Karen Bolitho,Kate Thodey,Susan E. Ledger,Judith H. Bowen,Jun-Hong Ma,Bhawana Nain,Daniel Cohen,Andrew P. Gleave,Ross N. Crowhurst,Bart J. Janssen,Jia-Long Yao,Richard D. Newcomb +14 more
TL;DR: This analysis suggests that the initial and final enzymatic steps with the biosynthetic pathways are important transcriptional regulation points for aroma production in apple.
Journal ArticleDOI
Apple ACC-oxidase and polygalacturonase: ripening-specific gene expression and promoter analysis in transgenic tomato.
Ross G. Atkinson,Karen Bolitho,Michele A. Wright,Teresa Iturriagagoitia-Bueno,Suzanne J. Reid,G. J. S. Ross +5 more
TL;DR: The fruit specificity of the apple ACC-oxidase and PG promoters was investigated in transgenic tomato plants using a nested set of promoter fragments fused to the β-glucuronidase (gusA) reporter gene.