Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis

Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.

https://www.jbc.org/content/252/3/1102.full.pdf

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

http://www.jbc.org/content/247/10/3242.full.pdf

Amino-terminal Sequence Analysis of Proteins Purified on a Nanomole Scale by Gel Electrophoresis

3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate

The unusual guanosine nucleotides, MSI and MSII, accumulated in vivo during amino-acid starvation of stringent strains of E. coli are shown to be synthesized in vitro as a product of an idling step in protein synthesis occurring on the ribosomes.

Klaus Weber

Papers

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis

Amino-terminal Sequence Analysis of Proteins Purified on a Nanomole Scale by Gel Electrophoresis

3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate

MSI and MSII made on ribosome in idling step of protein synthesis.