Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.

https://www.jbc.org/content/252/3/1102.full.pdf

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

The Regulation of Rabbit Skeletal Muscle Contraction I. BIOCHEMICAL STUDIES OF THE INTERACTION OF THE TROPOMYOSIN-TROPONIN COMPLEX WITH ACTIN AND THE PROTEOLYTIC FRAGMENTS OF MYOSIN

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis

3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

A new method is described for maximizing the yield of A-protein per liter of crude lysate. It involves a modified growth procedure for bacteriophage R17 which allows titers in excess of 1013 plaque forming units per ml, and a new procedure for purifying the A-protein by acetic acid treatment of the whole phage.

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1970.tb01134.x

Large Scale Purification of A‐Protein from Bacteriophage R17

Amino terminal peptides of RNA phage proteins synthesized in the cell free system.

Microtubules - immunofluorescence - evolution - antibody - sperm Antibody against tubulin from porcine brain was used to evaluate the immunological cross reactivity of tubulin from a variety of animal and plant cells. Indirect immunofluorescence microscopy revealed microtubule-containing structures including cytoplasmic microtubules, spindle microtubules, cilia and fIagella. Thus tubulin from diverse species of both mammals and plants show immunological cross-reactivity with tubulin from porcine brain. Results obtained by immunofluorescence microscopy are whenever possible compared with previously known ultrastructural results obtained by electron microscopy.

/pdf/identification-of-microtubular-structures-in-diverse-plant-4003jzi5di.pdf

Identification of microtubular structures in diverse plant and animal cells by immunological cross-reaction revealed in immunofluorescence microscopy using antibody against tubulin from porcine brain Darstellung von Mikrotubuli-Strukturen in verschiedenen Pflanzen- und Tierzellen durch immunologische Kreuzreaktion mit Hilfe der Immunfluoreszenzmikroskopie

Mary Osborn

Papers

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis

3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

Large Scale Purification of A‐Protein from Bacteriophage R17

Amino terminal peptides of RNA phage proteins synthesized in the cell free system.