Lan-Ying Lee

TL;DR: The protoplasts generated by this new Tape-Arabidopsis Sandwich method are suitable for the same range of research applications as those that use the current method, but require less operator skill, equipment and time.

TL;DR: A series of vectors for easy construction of N- terminal and C-terminal fusions of the target protein to the yellow spectral variant of the green fluorescent protein fragments are designed, demonstrating the utility of these vectors for the analysis of specific protein-protein interactions in various cellular compartments, including the nucleus, plasmodesmata, and chloroplasts of different plant species and cell types.

TL;DR: The history of development of T-DNA binary vector systems, and important components of these systems are described, which opened up the field of plant genetic engineering to numerous laboratories.

TL;DR: Multi-color BiFC is a useful technique to determine interactions simultaneously between a given" bait" protein and multiple "prey" proteins in living plant cells.

TL;DR: Improved the process of protoplast isolation and transfection of several plant species and developed a method to regenerate single mutagenized Nicotianna tabacum protoplasts into mature plants for targeted mutagenesis of the phytoene desaturase gene.