M
Martin Poenie
Researcher at University of Texas at Austin
Publications - 54
Citations - 27794
Martin Poenie is an academic researcher from University of Texas at Austin. The author has contributed to research in topics: Calcium & Microtubule organizing center. The author has an hindex of 31, co-authored 54 publications receiving 27373 citations. Previous affiliations of Martin Poenie include Duke University & University of California, San Diego.
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A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
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Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths.
TL;DR: Fura-2 as mentioned in this paper is a fluorescent Ca2+ indicator that can be used to estimate the ratio of Ca2-bound dye to free dye and hence cytosolic free Ca2+, which largely cancels out the effects of cell thickness, dye content, or instrumental efficiency.
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Requirement for intrinsic protein tyrosine kinase in the immediate and late actions of the EGF receptor.
William S. Chen,Cheri S. Lazar,Martin Poenie,Roger Y. Tsien,Gordon N. Gill,Michael G. Rosenfeld +5 more
TL;DR: It is concluded that the tyrosine kinase activity of the EGF receptor is essential for the diverse biochemical effects of EGF, including rapid alterations in intracellular calcium, activation of gene transcription, receptor down-regulation and the ultimate stimulatory effects on cell proliferation.
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Changes of free calcium levels with stages of the cell division cycle
TL;DR: In addition to the known transient rise of [Ca2+]i at fertilization, further peaks are now revealed during pronuclear migration, nuclear envelope breakdown, the metaphase/anaphase transition and cleavage.
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Calcium rises abruptly and briefly throughout the cell at the onset of anaphase
TL;DR: The close temporal association of the [Ca2+]i transient with the onset of anaphase suggests that calcium may have a signaling role in this event.