A new generation of Ca2+ indicators with greatly improved fluorescence properties.
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A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.About:
This article is published in Journal of Biological Chemistry.The article was published on 1985-03-25 and is currently open access. It has received 21582 citations till now. The article focuses on the topics: Fluorophore & Fluorescence.read more
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Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Atsushi Miyawaki,Juan Llopis,Roger Heim,J. Michael McCaffery,Joseph A. Adams,Mitsuhiko Ikura,Mitsuhiko Ikura,Roger Y. Tsien +7 more
TL;DR: New fluorescent indicators for Ca2+ that are genetically encoded without cofactors and are targetable to specific intracellular locations are constructed and dubbed ‘cameleons’.
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Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.
TL;DR: The results suggest that thapsigargin increases the concentration of cytosolic free Ca2+ in sensitive cells by an acute and highly specific arrest of the endoplasmic reticulum Ca 2+ pump, followed by a rapid Ca2+.
References
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Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.
TL;DR: Quin2 is a tetracarboxylic acid which binds Ca2+ with 1:1 stoichiometry and an effective dissociation constant of 115 nM in a cationic background mimicking cytoplasm.
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New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures.
TL;DR: The compounds described are fluorescent Ca2+ indicators absorbing in the ultraviolet region; the very large spectral shifts observed on binding Ca2+, fit the prediction that complexation should hinder the conjugation of the nitrogen lone-pair electrons with the aromatic rings.
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A non-disruptive technique for loading calcium buffers and indicators into cells.
TL;DR: A simple technique which loads Ca2+-selective chelators into the cytoplasm of intact cells in suspension and avoids the disadvantages of previous methods is described.
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Cytoplasmic pH and free Mg2+ in lymphocytes.
TL;DR: Measurements have been made of cytoplasmic pH, (pHi) and free Mg2+ concentration, ( [Mg2 +]i), in pig and mouse lymphocytes, and Mitogenic concentrations of concanavalin A (Con A) had no measurable effect on pH in the first hour.