M
Mats G. L. Gustafsson
Researcher at Howard Hughes Medical Institute
Publications - 35
Citations - 11902
Mats G. L. Gustafsson is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Microscopy & Microscope. The author has an hindex of 24, co-authored 35 publications receiving 10816 citations. Previous affiliations of Mats G. L. Gustafsson include University of California, San Francisco.
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Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
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Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
TL;DR: Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.
Journal ArticleDOI
Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination
Mats G. L. Gustafsson,Lin Shao,Peter M. Carlton,C. J. Rachel Wang,Inna N. Golubovskaya,W. Zacheus Cande,David A. Agard,David A. Agard,John W. Sedat +8 more
TL;DR: This work describes how spatially structured illumination microscopy can be applied in three dimensions to double the axial as well as the lateral resolution, with true optical sectioning, and has produced the first light microscopy images of the synaptonemal complex in which the lateral elements are clearly resolved.
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Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Lothar Schermelleh,Peter M. Carlton,Sebastian Haase,Lin Shao,Lukman Winoto,Peter Kner,Brian Burke,M. Cristina Cardoso,David A. Agard,Mats G. L. Gustafsson,Heinrich Leonhardt,John W. Sedat +11 more
TL;DR: Three-dimensional structured illumination microscopy (3D-SIM) opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
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Super-Resolution Video Microscopy of Live Cells by Structured Illumination
Peter Kner,Bryant B. Chhun,Eric R. Griffis,Lukman Winoto,Mats G. L. Gustafsson,Mats G. L. Gustafsson +5 more
TL;DR: A high-speed structured-illumination microscope capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points is demonstrated by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.