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Stefan Werner
Researcher at Martin Luther University of Halle-Wittenberg
Publications - 49
Citations - 3214
Stefan Werner is an academic researcher from Martin Luther University of Halle-Wittenberg. The author has contributed to research in topics: Golden Gate Cloning & Synthetic biology. The author has an hindex of 20, co-authored 48 publications receiving 2579 citations. Previous affiliations of Stefan Werner include Princeton University.
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A Modular Cloning System for Standardized Assembly of Multigene Constructs
TL;DR: A hierarchical modular cloning system that allows the creation at will and with high efficiency of any eukaryotic multigene construct, starting from libraries of defined and validated basic modules containing regulatory and coding sequences.
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A Golden Gate Modular Cloning Toolbox for Plants
Carola Engler,Mark Youles,Ramona Gruetzner,Tim-Martin Ehnert,Stefan Werner,Jonathan D. G. Jones,Nicola J. Patron,Sylvestre Marillonnet +7 more
TL;DR: A versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation is presented.
Journal ArticleDOI
Assembly of Designer TAL Effectors by Golden Gate Cloning
TL;DR: This work presents here a strategy for engineering of TALE proteins with novel DNA binding specificities based on the 17.5 repeat-containing AvrBs3 TALE as a scaffold, and assembled designer TALEs with new target specificities and tested their function in vivo.
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Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system
TL;DR: New features of this cloning system are presented that allow to increase the speed of assembly of multigene constructs and should be useful for generating the multiple construct variants that will be required for developing gene networks encoding novel functions, and fine-tuning the expression levels of the various genes involved.
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The role of fungal appressoria in plant infection.
TL;DR: Current knowledge on appressorium differentiation and function is summarized, aspects of initial stages of fungal development in the plant are discussed, and parts of signal transduction pathways involved in infection-related morphogenesis and virulence or pathogenicity are discussed.