M
Mark Youles
Researcher at University of East Anglia
Publications - 12
Citations - 1300
Mark Youles is an academic researcher from University of East Anglia. The author has contributed to research in topics: Synthetic biology & Effector. The author has an hindex of 6, co-authored 9 publications receiving 1021 citations. Previous affiliations of Mark Youles include Norwich Research Park & Medical Research Council.
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Journal ArticleDOI
A Golden Gate Modular Cloning Toolbox for Plants
Carola Engler,Mark Youles,Ramona Gruetzner,Tim-Martin Ehnert,Stefan Werner,Jonathan D. G. Jones,Nicola J. Patron,Sylvestre Marillonnet +7 more
TL;DR: A versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation is presented.
Journal ArticleDOI
Standards for plant synthetic biology: a common syntax for exchange of DNA parts.
Nicola J. Patron,Nicola J. Patron,Diego Orzaez,Sylvestre Marillonnet,Heribert Warzecha,Colette Matthewman,Colette Matthewman,Mark Youles,Oleg Raitskin,Oleg Raitskin,Aymeric Leveau,Gemma Farré,Christian Rogers,Alison G. Smith,Julian M. Hibberd,Alex A. R. Webb,James C. W. Locke,Sebastian Schornack,Jim Ajioka,David C. Baulcombe,Cyril Zipfel,Sophien Kamoun,Jonathan D. G. Jones,Hannah Kuhn,Silke Robatzek,H. Peter van Esse,Dale Sanders,Dale Sanders,Giles E. D. Oldroyd,Giles E. D. Oldroyd,Cathie Martin,Cathie Martin,Robert A. Field,Robert A. Field,Sarah E. O'Connor,Sarah E. O'Connor,Samantha Fox,Brande B. H. Wulff,Ben Miller,Andy Breakspear,Guru V. Radhakrishnan,Pierre-Marc Delaux,Dominique Loqué,Dominique Loqué,Antonio Granell,Alain Tissier,Patrick M. Shih,Thomas P. Brutnell,W. Paul Quick,Heiko Rischer,Paul D. Fraser,Asaph Aharoni,Christine A. Raines,Paul F. South,Jean-Michel Ané,Björn Hamberger,Jane A. Langdale,Jens Stougaard,Harro J. Bouwmeester,Michael K. Udvardi,James A. H. Murray,Vardis Ntoukakis,Patrick Schäfer,Katherine J. Denby,Keith J. Edwards,Anne Osbourn,Anne Osbourn,Jim Haseloff +67 more
TL;DR: A standard for Type IIS restriction endonuclease-mediated assembly is described, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units.
Journal ArticleDOI
Structural basis of hepatocyte growth factor/scatter factor and MET signalling.
Ermanno Gherardi,Sara Sandin,Maxim V. Petoukhov,John T. Finch,Mark Youles,Lars-Göran Öfverstedt,Ricardo Núñez Miguel,Tom L. Blundell,George F. Vande Woude,Ulf Skoglund,Dmitri I. Svergun +10 more
TL;DR: The study shows how the proteolytic mechanism of activation of the complex proteinases has been adapted to cell signaling in vertebrate organisms, offers a description of monomeric and dimeric ligand-receptor complexes, and provides a foundation to the structural basis of HGF/SF-MET signaling.
Journal ArticleDOI
Functional map and domain structure of MET, the product of the c-met protooncogene and receptor for hepatocyte growth factor/scatter factor
Ermanno Gherardi,Mark Youles,Ricardo Núñez Miguel,Tom L. Blundell,L. Iamele,Julian Gough,A. Bandyopadhyay,G. Hartmann,P.J.G. Butler +8 more
TL;DR: 3D models and a functional map of the large ectodomain of MET are provided and they have broad implications for structure-function of the MET receptor and the related semaphorin and plexin proteins.
Journal ArticleDOI
Engineering the NK1 fragment of hepatocyte growth factor/scatter factor as a MET receptor antagonist.
Mark Youles,Oliver Holmes,Maxim V. Petoukhov,Merel A. Nessen,Simona Stivala,Dmitri I. Svergun,Ermanno Gherardi +6 more
TL;DR: A subset of mutants at the NK1 dimer interface, such as the linker mutants Y124A or N127A or the kringle mutant V140A:I142A, bind the MET receptor with affinities comparable to wild-type NK1 but fail to assemble a dimeric, signalling competent NK1-MET complex.