Jilin University

About: Jilin University is a education organization based out in Changchun, China. It is known for research contribution in the topics: Catalysis & Apoptosis. The organization has 101453 authors who have published 88966 publications receiving 1444456 citations. The organization is also known as: Jílín Dàxué.

Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering.

Abstract: This paper reports a novel immunoassay based on surface-enhanced Raman scattering (SERS) and immunogold labelling with silver staining enhancement. Immunoreactions between immunogold colloids modified by a Raman-active probe molecule (e.g., 4-mercaptobenzoic acid) and antigens, which were captured by antibody-assembled chips such as silicon or quartz, were detected via SERS signals of Raman-active probe molecule. All the self-assembled steps were subjected to the measurements of ultraviolet-visible (UV-vis) spectra to monitor the formation of a sandwich structure onto a substrate. The immunoassay was performed by a sandwich structure consisting of three layers. The first layer was composed of immobilized antibody molecules of mouse polyclonal antibody against Hepatitis B virus surface antigen (PAb) on a silicon or quartz substrate. The second layer was the complementary Hepatitis B virus surface antigen (Antigen) molecules captured by PAb on the substrate. The third layer was composed of the probe-labelling immunogold nanoparticles, which were modified by mouse monoclonal antibody against Hepatitis B virus surface antigen (MAb) and 4-mercaptobenzoic acid (MBA) as the Raman-active probe on the surface of gold colloids. After silver staining enhancement, the antigen is identified by a SERS spectrum of MBA. A working curve of the intensity of a SERS signal at 1585 cm−1 due to the ν8a aromatic ring vibration of MBA versus the concentration of analyte (Antigen) was obtained and the non-optimized detection limit for the Hepatitis B virus surface antigen was found to be as low as 0.5 µg mL−1.

Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.

Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the specific cell and organ. An effective and very fast CRISPR/Cas9 genome editing in vitro and in vivo enabled by bioreducible lipid/Cas9 messenger RNA (mRNA) nanoparticle is reported. BAMEA-O16B, a lipid nanoparticle integrated with disulfide bonds, can efficiently deliver Cas9 mRNA and sgRNA into cells while releasing RNA in response to the reductive intracellular environment for genome editing as fast as 24 h post mRNA delivery. It is demonstrated that the simultaneous delivery of Cas9 mRNA and sgRNA using BAMEA-O16B knocks out green fluorescent protein (GFP) expression of human embryonic kidney cells with efficiency up to 90%. Moreover, the intravenous injection of BAMEA-O16B/Cas9 mRNA/sgRNA nanoparticle effectively accumulates in hepatocytes, and knocks down proprotein convertase subtilisin/kexin type 9 level in mouse serum down to 20% of nontreatment. The leading lipid nanoparticle, BAMEA-O16B, represents one of the most efficient CRISPR/Cas9 delivery nanocarriers reported so far, and it can broaden the therapeutic promise of mRNA and CRISPR/Cas9 technique further.

Activatable Hyaluronic Acid Nanoparticle as a Theranostic Agent for Optical/Photoacoustic Image-Guided Photothermal Therapy

Abstract: Photothermal therapy (PTT) is an emerging treatment modality that is under intensive preclinical investigations for the treatment of various medical conditions, including cancer. However, the lack of targeting function of PTT agents hampers its clinical application. An effective and nontoxic delivery vehicle that can carry PTT agents into tumor areas is still needed urgently. In this study, we developed a multifunctional nanocomposite by loading copper sulfide (CuS) into Cy5.5-conjugated hyaluronic acid nanoparticles (HANP), obtaining an activatable Cy5.5–HANP/CuS (HANPC) nanocomposite. In this system, Cy5.5 fluorescent signal is quenched by CuS inside the particle until the whole nanocomposite is degraded by hyaluronidase present in tumor, giving strong fluorescence signals delineating the tumor. Importantly, CuS with strong NIR absorbance appears to be an excellent contrast agent for photoacoustic (PA) imaging and an effective PTT agent. After intravenous administration of HANPC into SCC7 tumor-bearing ...

Branched polyethylenimine grafted electrospun polyacrylonitrile fiber membrane: a novel and effective adsorbent for Cr(VI) remediation in wastewater

Abstract: Cr(VI) is a well-known highly toxic metal ion and a thorough cleanup of this pollutant in wastewater is of special concern. Herein, a novel branched polyethylenimine (bPEI) grafted electrospun polyacrylonitrile (PAN) fiber membrane for Cr(VI) remediation based on adsorption was synthesized by a facile refluxing approach. Benefiting from the electrospinning process, the diameter of PAN fibers could be decreased to hundreds of nanometers from more than ten micrometers, which led to a higher adsorption capacity toward Cr(VI). In addition, the electrospun fibers showed good membrane forming properties, guaranteeing their application in the filtration purification process. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis, contact angle and so forth were used to characterize the grafted fiber membranes. Different grafting ratios were achieved by varying the refluxing time to search for the optimized removal capacity toward Cr(VI). The adsorption properties of the grafted fiber membrane including batch adsorption and filtration adsorption were investigated. The results revealed that the bPEI grafted electrospun PAN fiber adsorbent possessed an excellent adsorption capacity toward Cr(VI) (qm = 637.46 mg g−1), which was higher than many other adsorbents. Moreover, the batch adsorption and dynamic filtration could also make the Cr(VI) concentration notably decrease from 10 or 5 mg L−1 to below 0.05 mg L−1, which is recommended as the drinking water standard by WHO. The obtained results suggested that the grafted electrospun fiber membrane could be potentially applied to the efficient removal of Cr(VI) in wastewater treatment.