Journal ArticleDOI
Agrobacterium-mediated transformation of Nicotiana attenuata, a model ecological expression system
TLDR
An Agrobacterium-mediated transformation system for the model ecological expression system, Nicotiana attenuata, a native tobacco that occupies the post-fire niche in the Great Basin Desert of North America is presented.Abstract:
Research into the genetic basis of the ecological sophistication of plants is hampered by the availability of transformable systems with a wealth of well-described ecological interactions. We present an Agrobacterium-mediated transformation system for the model ecological expression system, Nicotiana attenuata, a native tobacco that occupies the post-fire niche in the Great Basin Desert of North America. We describe a transformation vector and a transformation procedure that differs from the standard cultivated tobacco transformation protocols in its use of selectable markers, explants, media and cultivation conditions. We illustrate its utility in the transformations with genes coding for key enzymes in the oxylipin cascade (lipoxygenase, allene oxide synthase, hydroperoxide lyase) in antisense orientations and present high-throughput screens useful for the detection of altered phenotypes for the oxylipin cascade (green leaf volatiles and jasmonic acid after wounding).read more
Citations
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Nicotine's defensive function in nature.
TL;DR: It is demonstrated that silencing pmt inhibits nicotine production, while the excess NA dimerizes to form anatabine, providing strong evidence that nicotine functions as an efficient defense in nature and highlights the value of transgenic techniques for ecological research.
Journal ArticleDOI
A novel class of gibberellin 2-oxidases control semidwarfism, tillering, and root development in rice.
Shuen-Fang Lo,Show-Ya Yang,Ku-Ting Chen,Yue-ie C. Hsing,Jan A. D. Zeevaart,Liang-Jwu Chen,Su-May Yu +6 more
TL;DR: It is shown that members of the rice (Oryza sativa) GA2ox family are differentially regulated and act in concert or individually to control GA levels during flowering, tillering, and seed germination and that improvements in plant architecture could be induced by overexpression of wild-type or modified C20 GA 2oxs.
Journal ArticleDOI
Priming of plant defense responses in nature by airborne signaling between Artemisia tridentata and Nicotiana attenuata.
TL;DR: This study demonstrates priming of plant defense responses as a mechanism of plant–plant signaling in nature, and provides an example for the analysis of between-plant signaling under ecologically realistic conditions.
Journal ArticleDOI
Antisense LOX expression increases herbivore performance by decreasing defense responses and inhibiting growth-related transcriptional reorganization in Nicotiana attenuata
Rayko Halitschke,Ian T. Baldwin +1 more
TL;DR: It is demonstrated that the complex phenotypic changes that mediate herbivore resistance are controlled by a specific part of the oxylipin cascade.
Journal ArticleDOI
Shared signals -'alarm calls' from plants increase apparency to herbivores and their enemies in nature.
TL;DR: In native populations and laboratory Y-tube choice experiments with transgenic Nicotiana attenuata plants unable to release particular volatiles, it is demonstrated that predatory bugs use terpenoids and green leafvolatiles to locate their prey on herbivore-attacked plants.
References
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Toshio Murashige,Folke Skoog +1 more
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Journal ArticleDOI
Construct design for efficient, effective and high-throughput gene silencing in plants.
S. Varsha Wesley,Chris A. Helliwell,Neil A. Smith,Ming-Bo Wang,Dean T. Rouse,Qing Liu,Paul S. Gooding,Surinder P. Singh,David Abbott,Peter A. Stoutjesdijk,Simon P. Robinson,Andrew P. Gleave,Allan Green,Peter M. Waterhouse +13 more
TL;DR: In this article, the authors examined design rules for efficient gene silencing, in terms of both the proportion of independent transgenic plants showing silencing and the degree of silencing.
Journal Article
Construct design for efficient, effective and high-throughput gene silencing in plants
S. Varsha Wesley,Chris A. Helliwell,Neil A. Smith,Ming-Bo Wang,Dean T. Rouse,Qing Liu,Paul S. Gooding,Surinder P. Singh,David Abbott,Peter A. Stoutjesdijk,Simon P. Robinson,Andrew P. Gleave,Allan Green,Peter M. Waterhouse +13 more
TL;DR: A generic vector is made that allows a simple, single PCR product from a gene of interest to be easily converted into a highly effective ihpRNA silencing construct, and a high-throughput vector that should facilitate the cloning of gene libraries or large numbers of defined genes, such as those in EST collections, using an in vitro recombinase system.
Journal ArticleDOI
Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.
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Antisense LOX expression increases herbivore performance by decreasing defense responses and inhibiting growth-related transcriptional reorganization in Nicotiana attenuata
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