Journal ArticleDOI
Clinical multiphoton tomography
TLDR
This review reflects state of the art technology and reports on applications in the fields of early stage melanoma detection, skin aging, nanoparticle imaging, tissue engineering, and in situ screening of pharmaceutical and cosmetical products.Abstract:
Clinical multiphoton tomography and two-photon microendoscopy provide clinicians and researchers with high-resolution in vivo optical biopsies based on two-photon autofluorescence, second harmonic generation, and fluorescence lifetime imaging. This review reflects state of the art technology and reports on applications in the fields of early stage melanoma detection, skin aging, nanoparticle imaging, tissue engineering, and in situ screening of pharmaceutical and cosmetical products. So far, more than 500 patients and volunteers in Europe, Asia, and Australia have been investigated with these novel molecular imaging tools. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)read more
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Journal ArticleDOI
Fluorescence Lifetime Measurements and Biological Imaging
TL;DR: The lifetime of a photophysical process is the time required by a population of N electronically excited molecules to be reduced by a factor of e via the loss of energy through fluorescence and other non-radiative processes and the average length of time τ is called the mean lifetime, or simply lifetime.
Journal ArticleDOI
Fluorescence lifetime imaging – techniques and applications
TL;DR: This paper describes the most frequent FLIM applications: Measurement of molecular environment parameters, protein‐interaction measurements by Förster resonance energy transfer (FRET), and measurements of the metabolic state of cells and tissue via their autofluorescence.
Journal ArticleDOI
Long-Lived Emissive Probes for Time-Resolved Photoluminescence Bioimaging and Biosensing
TL;DR: The design and applications of various kinds of long-lived emissive probes for bioimaging and biosensing via time-resolved photoluminescence techniques are summarized and the imaging contrast and sensing sensitivity are remarkably improved.
Journal ArticleDOI
Molecular Imaging: Current Status and Emerging Strategies
TL;DR: Current preclinical findings and advances in instrumentation suggest that these molecular imaging methods have numerous potential clinical applications and will be translated into clinical use in the near future.
Journal ArticleDOI
Probing Ferroelectrics Using Optical Second Harmonic Generation
TL;DR: In this paper, the optical second harmonic generation (SHG) was used to probe ferroelectric complex oxide crystals and thin films, and the results showed the ability to reveal domain structures and phases not normally visible with linear optics.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
Multiphoton microscopy in life sciences
TL;DR: Owing to the high NIR penetration depth, non‐invasive optical biopsies can be obtained from patients and ex vivo tissue by morphological and functional fluorescence imaging of endogenous fluorophores such as NAD(P)H, flavin, lipofuscin, porphyrins, collagen and elastin.
Journal ArticleDOI
Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin.
TL;DR: The spectroscopic data suggest that reduced pyridine nucleotides, NAD(P)H, are the primary source of the skin autofluorescence at 730 nm excitation, and that this conjecture is confirmed by the observation of the super-quadratic dependence of the fluorescence intensity on the excitation power.
Journal ArticleDOI
Video-rate confocal scanning laser microscope for imaging human tissues in vivo.
TL;DR: A video-rate confocal scanning laser microscope for reflectance imaging of human skin and oral mucosa in vivo with comparable resolution and contrast to that of conventional microscopy of excised biopsies (histology).
Journal ArticleDOI
In vivo assessment of human skin aging by multiphoton laser scanning tomography
TL;DR: The current findings are the first in vivo demonstration of this relationship, and show that specific characteristics of aged skin such as the ratio of extracellular matrix components collagen and elastin can be evaluated by in vivo AF and SHG measurements using near-IR femtosecond laser pulses.
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