Journal ArticleDOI
Determination of hydroxyproline.
TLDR
A critical review is given of hydroxyproline analysis, finding a procedure which is faster and more sensitive than the former one and as reliable.About:
This article is published in Clinica Chimica Acta.The article was published on 1967-11-01. It has received 1731 citations till now.read more
Citations
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Journal ArticleDOI
Aminoguanidine prevents diabetes-induced arterial wall protein cross-linking
TL;DR: The identification of aminoguanidine as an inhibitor of advanced nonenzymatic glycosylation product formation makes possible precise experimental definition of the pathogenetic significance of this process and suggests a potential clinical role for aminogsuanidine in the future treatment of chronic diabetic complications.
Journal ArticleDOI
A simplified method for the analysis of hydroxyproline in biological tissues.
TL;DR: The modified hydroxyproline assay presented in this communication will be useful for routine measurement of collagen content in extracts of various tissue specimens and can be used for batch processing of column fractions to monitor the collagen concentrations during purification.
Journal ArticleDOI
Adenovector-mediated gene transfer of active transforming growth factor-beta1 induces prolonged severe fibrosis in rat lung.
TL;DR: It is demonstrated that transient overexpression of active, but not latent, TGF-beta1 resulted in prolonged and severe interstitial and pleural fibrosis characterized by extensive deposition of the extracellular matrix proteins collagen, fibronectin, and elastin and by emergence of cells with the myofibroblast phenotype.
Journal ArticleDOI
Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels.
Robert L. Mauck,Michael A. Soltz,Christopher C.-B. Wang,Dennis D. Wong,Pen-Hsiu Grace Chao,Wilmot B. Valhmu,Clark T. Hung,Gerard A. Ateshian +7 more
TL;DR: The hypothesis that the application of dynamic deformational loading at physiological strain levels enhances chondrocyte matrix elaboration in cell-seeded agarose scaffolds to produce a more functional engineered tissue construct than in free swelling controls is tested.
Journal ArticleDOI
Long-term prevention of postmenopausal osteoporosis by œstrogen
TL;DR: In this paper, the bone mineral content of oophorectomised women with Stochastic Hormone Replacement (SHR) was found to increase bone mineral levels during the first three years of treatment.
References
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Journal ArticleDOI
The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.
TL;DR: Method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins, and significant amounts of hydroXYproline were found in crystalline preparations of pepsin, elastase.
Journal ArticleDOI
Two Improved and Simplified Methods for the Spectrophotometric Determination of Hydroxyproline.
Imanuel. Bergman,Roy Loxley +1 more
Journal ArticleDOI
The determination of hydroxyproline.
Robert E. Neuman,Milan A. Logan +1 more
TL;DR: A short and simple calorimetric method is here described that is applicable to the determination of hydroxyproline in hydrolysates of 40 to 100 y of collagen with a reproducibility of f2 per cent and an accuracy of f1 per cent as judged by recovery of hydroXYproline from elastin hydrolysate and from an amino acid mixture simulating collagen.