Open AccessJournal Article
Protein Measurement with the Folin Phenol Reagent
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TLDR
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.About:
This article is published in Journal of Biological Chemistry.The article was published on 1951-11-01 and is currently open access. It has received 289852 citations till now. The article focuses on the topics: Reagent & Sulfhydryl reagent.read more
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.
TL;DR: The purification of homogeneous glutathione S-transferases B and C from rat liver is described, and only transferases A and C are immunologically related.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
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The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
References
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Journal ArticleDOI
A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum
TL;DR: A rapid method has been devised which requires only 5 c.mm.
Journal ArticleDOI
On tyrosine and tryptophane determinations in proteins
Otto Folin,Vintila Ciocalteu +1 more
TL;DR: In this article, the authors summarized research into the existing methods for the quantitative determination of tyrosine and tryptophane in proteins, including the Folin-Looney method, which is based on reaction of a phosphotungstic phosphomolybdic acid in a phenol solution.
Journal ArticleDOI
On phosphotungstic-phosphomolybdic compounds as color reagents
Otto Folin,W. Denis +1 more