Journal ArticleDOI
Genome size and the proportion of repeated nucleotide sequence DNA in plants.
TLDR
It is concluded that most of the variation in nuclear DNA mass in higher plant chromosomes can be accounted for by variation in repeated-sequence DNA.Abstract:
The reannealing kinetics of denatured DNA fragments from 23 species of higher plants have been studied, using hydroxylapatite chromatography to distinguish reannealed from single-stranded DNA. The 2C nuclear DNA contents of the species varied between 1.7 and 98 pg. The proportions of DNA in species with a nuclear DNA mass above 5 pg that reannealed with the kinetics of sequences present in more than 100 copies were high (69–92% with a mean of 80±2.0%). For species with less than 4 pg of DNA, the mean proportion of repeated-sequence DNA was 62±2.9%. It is concluded that most of the variation in nuclear DNA mass in higher plant chromosomes can be accounted for by variation in repeated-sequence DNA. The consequences of altering the adapted DNA content of a species by the addition of families of repeated sequences are discussed in relation to the proportion of repeated-sequence DNA.read more
Citations
More filters
Journal ArticleDOI
Nested Retrotransposons in the Intergenic Regions of the Maize Genome
Phillip SanMiguel,Alexander Tikhonov,Young Kwan Jin,Natasha Motchoulskaia,Dmitrii Zakharov,Admasu Melake-Berhan,Patricia S. Springer,Keith J. Edwards,Michael Lee,Zoya V Avramova,Jeffrey L. Bennetzen +10 more
TL;DR: Diagnostic sequencing indicated that a 280-kilobase region containing the maize Adh1-F and u22 genes is composed primarily of retrotransposons inserted within each other, and ten retroelement families were discovered.
Journal ArticleDOI
A chromosome conformation capture ordered sequence of the barley genome
Martin Mascher,Heidrun Gundlach,Axel Himmelbach,Sebastian Beier,Sven Twardziok,Thomas Wicker,Volodymyr Radchuk,Christoph Dockter,Pete E. Hedley,Joanne Russell,Micha Bayer,Luke Ramsay,Hui Liu,Georg Haberer,Xiao-Qi Zhang,Qisen Zhang,Roberto A. Barrero,Lin Li,Stefan Taudien,Marco Groth,Marius Felder,Alex Hastie,Hana Šimková,Helena Staňková,Jan Vrána,Saki Chan,María Muñoz-Amatriaín,Rachid Ounit,Steve Wanamaker,Dan Bolser,Christian Colmsee,Thomas Schmutzer,Lala Aliyeva-Schnorr,Stefano Grasso,Jaakko Tanskanen,Anna Chailyan,Dharanya Sampath,Darren Heavens,Leah Clissold,Sujie Cao,Brett Chapman,Fei Dai,Yong Han,Hua Li,Xuan Li,Chongyun Lin,John K. McCooke,Cong Tan,Penghao Wang,Songbo Wang,Shuya Yin,Gaofeng Zhou,Jesse Poland,Matthew I. Bellgard,Ljudmilla Borisjuk,Andreas Houben,Jaroslav Doležel,Sarah Ayling,Stefano Lonardi,Paul J. Kersey,Peter Langridge,Gary J. Muehlbauer,Matthew D. Clark,Matthew D. Clark,Mario Caccamo,Mario Caccamo,Alan H. Schulman,Klaus F. X. Mayer,Matthias Platzer,Timothy J. Close,Uwe Scholz,Mats Hansson,Guoping Zhang,Ilka Braumann,Manuel Spannagl,Chengdao Li,Chengdao Li,Chengdao Li,Robbie Waugh,Robbie Waugh,Nils Stein,Nils Stein +81 more
TL;DR: The importance of the barley reference sequence for breeding is demonstrated by inspecting the genomic partitioning of sequence variation in modern elite germplasm, highlighting regions vulnerable to genetic erosion.
Journal ArticleDOI
The paleontology of intergene retrotransposons of maize
TL;DR: The structure of the adh1 region appears to be standard relative to the other gene-containing regions of the maize genome, thus suggesting that retrotransposon insertions have increased the size of the corn genome from approximately 1200 Mb to 2400 Mb in the last three million years.
Journal ArticleDOI
Genome Plasticity a Key Factor in the Success of Polyploid Wheat Under Domestication
Jorge Dubcovsky,Jan Dvorak +1 more
TL;DR: Wheat compensates for the diversity bottlenecks expected from recent domestication and polyploid speciation events by capturing part of the genetic diversity of its progenitors and by generating new diversity at a relatively fast pace.
Journal ArticleDOI
Transposable element contributions to plant gene and genome evolution.
TL;DR: Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function and may be responsible for the rate at which incompatibility is generated in separated populations.
References
More filters
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
Variation and Evolution in Plants.
TL;DR: JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive.
Journal ArticleDOI
Repeated Sequences in DNA
Roy J. Britten,D. E. Kohne +1 more
TL;DR: Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms and used in medicine, science, and engineering.