Highly Sensitive and Fast Protein Detection with Coomassie Brilliant Blue in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
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In this paper, the detection of proteins in SDS-PAGE is an important first step for protein analysis, and various experimentalefforts have been directed to develop and improve theprotein detection methods.Abstract:
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, KoreaReceived August 12, 2002Key Words : Coomasie brilliant blue, 2-Dimensional gel electrophoresis, ProteomicsDetection of proteins in sodium dodecyl sulfate-polyacryl-amide gel electrophoresis (SDS-PAGE) is an important firststep for protein analysis. For years, various experimentalefforts have been directed to develop and improve theprotein detection methodsread more
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References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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TL;DR: An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G‐250 and R‐250 to combine the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining.
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Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis
TL;DR: Convincing arguments for the colloidal properties of the CBB dyes are presented, formulating the rationale for intensified protein staining with CBBs dyes in polyacrylamide gels without background staining.
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Detection technologies in proteome analysis
TL;DR: New multiplexing capabilities should greatly enhance the applicability of the two-dimensional gel electrophoresis technique with respect to addressing fundamental questions related to proteome-wide changes in protein expression and post-translational modification.
Book
Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods
TL;DR: This chapter discusses Solubilization of Proteins in 2D Electrophoresis with Carrier Ampholytes and Troubleshooting for IPG and Horizontal PAGE.