Journal ArticleDOI
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
TLDR
Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.About:
This article is published in Journal of Chromatography A.The article was published on 1990-01-19. It has received 1761 citations till now. The article focuses on the topics: Hydrophilic interaction chromatography & Aqueous normal-phase chromatography.read more
Citations
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Journal ArticleDOI
Metabolite Profiling Identifies a Key Role for Glycine in Rapid Cancer Cell Proliferation
Mohit Jain,Roland Nilsson,Sonia Sharma,Nikhil Madhusudhan,Nikhil Madhusudhan,Toshimori Kitami,Toshimori Kitami,Amanda Souza,Ran Kafri,Marc W. Kirschner,Clary B. Clish,Vamsi K. Mootha,Vamsi K. Mootha +12 more
TL;DR: Glycine consumption and expression of the mitochondrial glycine biosynthetic pathway was identified as strongly correlated with rates of proliferation across cancer cells, and higher expression of this pathway was associated with greater mortality in breast cancer patients.
Journal ArticleDOI
Protein Analysis by Shotgun/Bottom-up Proteomics
TL;DR: The progress of proteomics has been driven by the development of new technologies for peptide/protein separation, mass spectrometry analysis, isotope labeling for quantification, and bioinformatics data analysis.
Journal ArticleDOI
Hydrophilic interaction chromatography.
Petrus Hemström,Knut Irgum +1 more
TL;DR: The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.
Journal ArticleDOI
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more
TL;DR: Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases and their applications for separations of polar compounds in complex matrices.
BookDOI
The protein protocols handbook
TL;DR: Sectional Contents Only: Quantitation of Proteins and Peptides and Detection in Gels.
References
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Journal ArticleDOI
Surface silanols in silica-bonded hydrocarbonaceous stationary phases : II. Irregular retention behavior and effect of silanol masking
TL;DR: In this paper, a dual retention mechanism that postulates solute retention as a result of both solvophobic (hydrophobic) and silanophilic interactions between the eluite and stationary phase was proposed.
Journal ArticleDOI
Prediction of peptide retention times in reversed-phase high-performance liquid chromatography I. Determination of retention coefficients of amino acid residues of model synthetic peptides
TL;DR: In this article, the authors examined the contribution of individual amino acid residues to peptide retention on reversed-phase (RP) columns by measuring their effect on retention of a model synthetic peptide: Ac-Gly-X-X-(Leu)3-(Lys)2-amide, where X is substituted by the 20 amino acids found in proteins.
Journal ArticleDOI
Liquid chromatography of saccharides
TL;DR: The analysis of saccharides by liquid chromatography on an automated instrument is described in this paper, where conditions for the resolution and quantification of fructose, glucose, sucrose, melibiose, raffinose, betaine and three kestose isomers as well as starch hydrolysates are given.
Journal ArticleDOI
Hydrophobic high-performance liquid chromatography of hormonal polypeptides and proteins on alkylsilane-bonded silica.
M.J. O'Hare,Edouard C. Nice +1 more
TL;DR: The separation of at least eighteen discrete components from a partially-purified posterior pituitary extract has demonstrated the capability of alkylsilane-type reversed-phase packings for the hydrophobic high-performance liquid chromatography of complex biological mixtures.
Journal ArticleDOI
Profiling of amino acids in body fluids and tissues by means of liquid chromatography
TL;DR: The needs of urgent diagnoses and the needs emerging from acute forms of diseases have directed progress in amino acid profiling to modern, rapid, automated analyses that can be done at reasonable cost.
Related Papers (5)
Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography.
Yong Guo,Sheetal Gaiki +1 more
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more