Journal ArticleDOI
Intracellular effect of ultrashort electrical pulses.
TLDR
The intracellular effect extends conventional electroporation to cellular substructures and opens the potential for new applications in apoptosis induction, gene delivery to the nucleus, or altered cell functions, depending on the electrical pulse conditions.Abstract:
A simple electrical model for biological cells predicts an increasing probability for electric field interactions with cell substructures of prokaryotic and eukaryotic cells when the electric pulse duration is reduced into the sub-microsecond range. The validity of this hypothesis was verified experimentally by applying electrical pulses with electric field intensities of up to 5.3 MV/m to human eosinophils in vitro. When 3-5 pulses of 60 ns duration were applied to human eosinophils, intracellular granules were modified without permanent disruption of the plasma membrane. In spite of the extreme electrical power levels applied to the cells thermal effects could be neglected because of the ultrashort pulse duration. The intracellular effect extends conventional electroporation to cellular substructures and opens the potential for new applications in apoptosis induction, gene delivery to the nucleus, or altered cell functions, depending on the electrical pulse conditions.read more
Citations
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Journal ArticleDOI
Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research
TL;DR: The theory of electroporation is outlined, the use of short high-voltage pulses to overcome the barrier of the cell membrane is described, and data on safety and efficacy is summed up on delivery of chemotherapy to tumours in cancer patients.
Patent
Methods and apparatus for renal neuromodulation
Mark Deem,Hanson S. Gifford,Denise Zarins,Douglas Sutton,Erik Thai,Mark Gelfand,Howard R. Levin +6 more
TL;DR: In this article, a pulsed electric field was used to induce electroporation or electrofusion of percutaneous intravascular vessels to reduce expansion of an acute myocardial infarction, reduce or prevent the onset of morphological changes that are affiliated with congestive heart failure, and/or be efficacious in the treatment of end stage renal disease.
Patent
Renal nerve stimulation method and apparatus for treatment of patients
Howard R. Levin,Mark Gelfand +1 more
TL;DR: A method and apparatus for treatment of heart failure, hypertension and renal failure by stimulating the renal nerve is described in this paper, where the goal is to reduce the sympathetic activity of renal nerve.
Journal ArticleDOI
Electroporation-Based Technologies for Medicine: Principles, Applications, and Challenges
TL;DR: This review describes the theory and current applications of electroporation in medicine and then discusses current challenges in Electroporation research and barriers to a more extensive spread of these clinical applications.
Journal ArticleDOI
Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition
TL;DR: The studies show that nsPEF effects are distinctly different than electroporation pulses and provide the first evidence for the potential application of nsPEf to induce apoptosis and inhibit tumor growth.
References
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Journal ArticleDOI
A review of short pulse generator technology
John J. Mankowski,M. Kristiansen +1 more
TL;DR: A detailed discussion of the various circuit designs and a list of commercially available high-voltage pulse generators can be found in this article, along with a brief description of the three primary switch types, reed, spark gap, and solid state.
Journal ArticleDOI
Thermal and nonthermal mechanisms of interaction of radio-frequency energy with biological systems
TL;DR: In this paper, thermal and non-thermal mechanisms of interaction between radiofrequency (RF) fields and biological systems, focusing on pulsed fields with high peak power but low duty cycle, are reviewed.
Journal Article
Distinctive cationic proteins of the human eosinophil granule: major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin.
Steven J. Ackerman,David A. Loegering,Per Venge,I. Olsson,J. B. Harley,A S Fauci,Gerald J. Gleich +6 more
TL;DR: Observations demonstrate that MBP, ECP, and EDN are distinctive cationic proteins of the human eosinophil granule and that eosInophilgranules from patients with eOSinophilia contain considerably greater quantities of MBP than ECP.
Journal ArticleDOI
Electroporation dynamics in biological cells subjected to ultrafast electrical pulses: a numerical simulation study.
TL;DR: A model analysis of electroporation dynamics in biological cells has been carried out based on the Smoluchowski equation and it is shown that irreversible breakdown would lead to the formation of a few large pores, while a large number of smaller pores would appear in the case of reversible breakdown.