Journal ArticleDOI
Polyamidoamine cascade polymers mediate efficient transfection of cells in culture
Jean Haensler,Francis C. Szoka +1 more
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It is shown, using luciferase and beta-galactosidase containing plasmids, that dendrimers mediate high efficiency transfection of a variety of suspension and adherent cultured mammalian cells.About:
This article is published in Bioconjugate Chemistry.The article was published on 1993-09-01. It has received 1255 citations till now. The article focuses on the topics: Dendrimer & Transfection.read more
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Design and development of polymers for gene delivery
TL;DR: With the growing understanding of polymer gene-delivery mechanisms and continued efforts of creative polymer chemists, it is likely that polymer-based gene-Delivery systems will become an important tool for human gene therapy.
Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis.
TL;DR: The magnitude of the cytotoxic effects of all polymers were found to be time- and concentration dependent and the molecular weight as well as the cationic charge density of the polycations were confirmed as key parameters for the interaction with the cell membranes and consequently the cell damage.
Journal ArticleDOI
Designing dendrimers for biological applications.
TL;DR: Advances in understanding of the role of molecular weight and architecture on the in vivo behavior of dendrimers, together with recent progress in the design of biodegradable chemistries, has enabled the application of these branched polymers as anti-viral drugs, tissue repair scaffolds, targeted carriers of chemotherapeutics and optical oxygen sensors.
Journal ArticleDOI
Synthetic DNA delivery systems.
Dan Luo,Saltzman Wm +1 more
TL;DR: The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology and rapid advances have recently been made in understanding of mechanisms for DNA stability and transport within cells.
References
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
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Firefly luciferase gene: structure and expression in mammalian cells.
TL;DR: Assaying the expression of luciferase provides a rapid and inexpensive method for monitoring promoter activity and is estimated to be from 30- to 1,000-fold more sensitive than assaying chloramphenicol acetyltransferase expression.
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Starburst Dendrimers: Molecular-Level Control of Size, Shape, Surface Chemistry, Topology, and Flexibility from Atoms to Macroscopic Matter
TL;DR: Starburst dendrimers are three-dimensional, highly ordered oligomeric and polymeric compounds formed by reiterative reaction sequences starting from smaller molecules—“initiator cores” such as ammonia or pentaerythritol.
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Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent
TL;DR: A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized and its N-hydroxysuccinimide ester group reacts with amino groups and the 2- pyridyl disulphide structure reacts with aliphatic thiols, resulting in a new thiolation procedure for proteins.