Journal ArticleDOI
Quantitative assessment of myocardial collagen with picrosirius red staining and circularly polarized light.
Reads0
Chats0
TLDR
Analysis using circularly polarized light has the ability to enhance histologic assessment of tissue and can provide additional insights into the composition and structure of myocardial collagen.Abstract:
Collagen plays a major role in the structural organization of the heart and therefore direct visualization of collagen fibers is a crucial component of cardiac analysis. Although linearly polarized light has proven an effective tool for the examination of myocardial collagen in histologic sections, the use of circularly polarized light may offer advantages and additional possibilities. We examined the potential enhancement of collagen analysis using circularly polarized light in two ways. We first measured the brightness, and hence indirectly assessed the birefringence, of collagen fibers in scars examined at different times after myocardial infarction. Secondly, we measured collagen content in myocardial tissue and compared results obtained from brightfield analysis of trichrome stained sections with those obtained from circularly polarized light analysis of picrosirius red stained sections. We observed a progressive increase in the maximum brightness of collagen fibers in the scar with time, and a time-dependent shift in the relative distribution of collagen fiber brightness from lower to higher levels. We found consistently lower values of collagen content in trichrome stained versus picrosirius red stained tissue, and concluded that trichrome staining underestimated collagen content. The information provided by these studies could not be obtained by brightfield analysis and could be only partially obtained from linearly polarized light analysis. Thus, analysis using circularly polarized light has the ability to enhance histologic assessment of tissue and can provide additional insights into the composition and structure of myocardial collagen.read more
Citations
More filters
Journal ArticleDOI
The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions
Matthias Nahrendorf,Filip K. Swirski,Elena Aikawa,Lars Stangenberg,Thomas Wurdinger,Jose-Luiz Figueiredo,Peter Libby,Peter Libby,Ralph Weissleder,Mikael J. Pittet +9 more
TL;DR: This work identifies two distinct phases of monocyte participation after MI and proposes a model that reconciles the divergent properties of these cells in healing and identifies new therapeutic targets that can influence healing and ventricular remodeling after MI.
Journal ArticleDOI
Pathophysiologically relevant concentrations of tumor necrosis factor- α promote progressive left ventricular dysfunction and remodeling in rats
Biykem Bozkurt,Scott B. Kribbs,Fred J. Clubb,Lloyd H. Michael,Vladimir V. Didenko,Peter J. Hornsby,Yukihiro Seta,Hakan Oral,Francis G. Spinale,Douglas L. Mann +9 more
TL;DR: These studies suggest that pathophysiologically relevant concentrations of TNF-alpha are sufficient to mimic certain aspects of the phenotype observed in experimental and clinical models of heart failure.
Journal ArticleDOI
Growth of Engineered Human Myocardium With Mechanical Loading and Vascular Coculture
Nathaniel L. Tulloch,Veronica Muskheli,Maria V. Razumova,F. Steven Korte,Michael Regnier,Kip D. Hauch,Lil Pabon,Hans Reinecke,Charles E. Murry +8 more
TL;DR: The results indicate that both mechanical load and vascular cell coculture control cardiomyocyte proliferation, and that mechanical load further controls the hypertrophy and architecture of engineered human myocardium.
Journal ArticleDOI
Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues
Russell A. Norris,Brook Damon,Vladimir Mironov,Vladimir Kasyanov,Anand Ramamurthi,Anand Ramamurthi,Ricardo A. Moreno-Rodriguez,Thomas C. Trusk,Jay D. Potts,Richard L. Goodwin,Jeffrey Davis,Stanley Hoffman,Xuejun Wen,Yukiko Sugi,Christine B. Kern,Corey H. Mjaatvedt,Debi Turner,Toru Oka,Simon J. Conway,Jeffery D. Molkentin,Gabor Forgacs,Roger R. Markwald +21 more
TL;DR: Functional biomechanical properties of periostin null skin specimens and atrioventricular valve explant experiments provided direct evidence of the role that periastin plays in regulating the viscoelastic properties of connective tissues.
Journal ArticleDOI
Prognostic Significance of Myocardial Fibrosis Quantification by Histopathology and Magnetic Resonance Imaging in Patients With Severe Aortic Valve Disease
Clerio F. Azevedo,Marcelo Nigri,Maria de Lourdes Higuchi,Pablo Maria Alberto Pomerantzeff,Guilherme Sobreira Spina,Roney Orismar Sampaio,Flávio Tarasoutchi,Max Grinberg,Carlos E. Rochitte +8 more
TL;DR: The amount of MF, either by histopathology or by ce-MRI, is associated with the degree of left ventricular functional improvement and all-cause mortality late after aortic valve replacement in patients with severe aortIC valve disease.
References
More filters
Journal ArticleDOI
Some statistical methods useful in circulation research.
TL;DR: Some statistical techniques for analyzing the kinds of studies typically reported in Circulation Research are described and particular emphasis is given to the comparison of means from more than two populations.
Journal ArticleDOI
Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections
TL;DR: Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen.
Journal ArticleDOI
Cardiac interstitium in health and disease: the fibrillar collagen network
TL;DR: Future studies into collagen gene regulation, gene switching events and the control of collagen synthesis and degradation are needed to develop a more complete understanding of the relation between the collagen network and acquired and inherited forms of heart disease.
Journal ArticleDOI
The use of the Picrosirius-polarization method for the study of the biopathology of collagen
Journal ArticleDOI
Polarization microscopic studies of connective tissue stained with picro-sirius red FBA.
TL;DR: Correlation of polarization microscopic observations with electron microscopic and x-ray diffraction data demonstrated relations between molecular orientation of connective tissue structures and birefringence.
Related Papers (5)
Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections
Differential staining of collagens type I, II and III by Sirius Red and polarization microscopy.
Ventricular remodeling after myocardial infarction. Experimental observations and clinical implications.
Marc A. Pfeffer,Eugene Braunwald +1 more