Randomly amplified polymorphic dna-a brief review
TLDR
The present communication gives emphasis on basic knowledge about RAPD, procedure, its advantages disadvantages, limitations and applications of RAPD.Abstract:
RAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotid es), resulting in the amplification of many discrete DNA . The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucl eotide sequence polymorphisms in a DNA amplification based assay using only a single prime r of arbitrary nucleotide sequence. The RAPD technology has provided a quick and efficient scree n for DNA-sequence polymorphisms at a very large no of loci. The present communication gives emphasis on basic knowledge about RAPD, procedure, its advantages disadvantages, limitations and applicati ons of RAPD.read more
Citations
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Molecular Markers: an Introduction and Applications
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Kareem A. Mosa,Kareem A. Mosa,Sanjay Gairola,Rahul A. Jamdade,Ali El-Keblawy,Khawla Ibrahim Al Shaer,Eman Khalid Al Harthi,Hatem A. Shabana,Tamer Mahmoud +8 more
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Population genetic structure and diversity analysis in Hedychium coronarium populations using morphological, phytochemical and molecular markers
Asit Ray,Asit Ray,Sudipta Jena,Tarun Haldar,Ambika Sahoo,Basudeba Kar,Jeetendranath Patnaik,Biswajit Ghosh,Pratap Chandra Panda,Namita Mahapatra,Sanghamitra Nayak +10 more
TL;DR: A high degree of molecular and phytochemical variation among H. coronarium populations in relation to geographic locations is revealed and can be exploited for conservation and breeding programmes of H. coronerarium for its genetic improvement.
Journal ArticleDOI
Molecular typing and differences in biofilm formation and antibiotic susceptibilities among Prototheca strains isolated in Italy and Brazil.
Stefano Morandi,Paola Cremonesi,Emanuele Capra,Tiziana Silvetti,Marilù Decimo,V. Bianchini,Ana Carolina Alves,Agueda Castagna de Vargas,G.M. Costa,Márcio Garcia Ribeiro,Milena Brasca +10 more
TL;DR: Results obtained in this study confirmed that RAPD-PCR is a rapid, inexpensive, and highly discriminating tool for Prototheca strains characterization and could give a good scientific contribution for better understanding the protothecal mastitis in dairy herd.
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RAPD analysis of Leuconostoc mesenteroides strains associated with vegetables and food products from Korea
TL;DR: The results showed that 16S rDNA sequencing and RAPD-PCR are suitable preliminary molecular tools for identification and characterization of bacteria.
References
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Bruce S. Weir,C. Clark Cockerham +1 more
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Arlequin (version 3.0): An integrated software package for population genetics data analysis
TL;DR: Arlequin ver 3.0 as discussed by the authors is a software package integrating several basic and advanced methods for population genetics data analysis, like the computation of standard genetic diversity indices, the estimation of allele and haplotype frequencies, tests of departure from linkage equilibrium, departure from selective neutrality and demographic equilibrium, estimation or parameters from past population expansions, and thorough analyses of population subdivision under the AMOVA framework.
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.
TL;DR: In this article, a framework for the study of molecular variation within a single species is presented, where information on DNA haplotype divergence is incorporated into an analysis of variance format, derived from a matrix of squared-distances among all pairs of haplotypes.