Journal ArticleDOI
Relationships between the cell volume and the carbon content of bacteria
N. D. Romanova,Andrey F. Sazhin +1 more
TLDR
The principal goal of the present study was searching for a generally applicable or methodology-dependent converting factor for the bacterial biomass calculation, which suggested a relationship generalized for different methods of carbon and cell volume recalculations and applicable for bacterial cells larger than 0.025 μm3.Abstract:
The bacterial biomass is an essential point in microbial ecology. The dry weight and carbon con� tent of microorganisms are traditionally used for the calculation of the fluxes of carbon and energy in aquatic ecosystems. Since direct measurements of these parameters in natural conditions are rather difficult, a certain biomass-carbon converting factor is used, which is determined experimentally or by empirical calculations. However, the relationship between the cell volume and its dry weight (in particular, carbon) does not depend only on the cell size and the conditions of its growth but also on the fixation and staining methods used. We made an attempt to summarize all the present data on the relationships between the cell volume, its dry weight, and the carbon content. Thus, the principal goal of the present study was searching for a generally applicable or methodologydependent converting factor for the bacterial biomass calculation. Thereto, all the data available were grouped according to the dye used, as well as to the methods of fixation and the dry weight determination. The data on the most frequent combinations of dies and fixatives are insufficient for precise calculations; therefore, we suggest a relationship generalized for different methods of carbon and cell volume recalculations and applicable for bacterial cells larger than 0.025 μm 3 , as fgC cell -1 = 133.754 × V 0.438 .read more
Citations
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Journal ArticleDOI
Global distribution of microbial abundance and biomass in subseafloor sediment
TL;DR: This work shows that total microbial cell abundance in subseafloor sediment varies between sites by ca.
Journal ArticleDOI
SAR11 Bacteria: The Most Abundant Plankton in the Oceans.
TL;DR: The study of SAR11 is providing insight into the biogeochemistry of labile DOM and is affecting microbiology beyond marine science by providing a model for understanding the evolution and function of streamlined cells.
Journal ArticleDOI
Microbial community dynamics alleviate stoichiometric constraints during litter decay
TL;DR: It is concluded that identifying and implementing microbial community-driven mechanisms in biogeochemical models are necessary for accurately predicting terrestrial C fluxes in response to changing environmental conditions.
Journal ArticleDOI
Life under extreme energy limitation: a synthesis of laboratory- and field-based investigations.
Mark A. Lever,Karyn L. Rogers,Karen G. Lloyd,Jörg Overmann,Bernhard Schink,Rudolf K. Thauer,Tori M. Hoehler,Bo Barker Jørgensen +7 more
TL;DR: Past investigations on microbial energy requirements and adaptations to energy limitation, identify gaps in current knowledge, and outline possible future foci of research on life under extreme energy limitation are discussed.
Journal ArticleDOI
Microbial temperature sensitivity and biomass change explain soil carbon loss with warming.
Tom W. N. Walker,Tom W. N. Walker,Christina Kaiser,Christina Kaiser,Florian Strasser,Craig W. Herbold,Niki I. W. Leblans,Niki I. W. Leblans,Dagmar Woebken,Ivan A. Janssens,Bjarni D. Sigurdsson,Andreas Richter,Andreas Richter +12 more
TL;DR: A framework is provided for interpreting the links between temperature, microbial activity and soil carbon loss on timescales relevant to Earth’s climate system via their control over microbial biomass.
References
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Journal ArticleDOI
Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton
TL;DR: Cellular carbon and nitrogen content and cell volume of nutritionally and morphologically diverse dinoflagellate species were measured to determine carbon to volume and nitrogen to volume relationships.
Journal ArticleDOI
Protein content and protein synthesis rates of planktonic marine bacteria
Meinhard Simon,Farooq Azam +1 more
TL;DR: Bacterial protein production method was an order of magnitude more sensitive and yielded bacterial carbon production directly without the need to know the cell size of the part of the assemblage in growth state.
BookDOI
HANDBOOK Of Methods in Aquatic Microbial Ecology
TL;DR: This work focuses on the isolation and culture of Marine Protists from Estuarine, Littoral, Psammolittoral and Sublittoral Waters and the identification, Enumeration, and Diversity of Anaerobic and Microaerobic Protists.
Journal ArticleDOI
Relationships between Biovolume and Biomass of Naturally Derived Marine Bacterioplankton.
Sanghoon Lee,Jed A. Fuhrman +1 more
TL;DR: It is concluded that natural marine bacterial biomass and production may be higher than was previously thought and that variations in bacterial size may not reflect variations in biomass per cell.
Journal ArticleDOI
Relationships between cell volume and the carbon and nitrogen content of marine photosynthetic nanoplankton
Peter G. Verity,Charles Y. Robertson,Craig R. Tronzo,Melinda G. Andrews,James R. Nelson,Michael E. Sieracki +5 more
TL;DR: Unialgal cultures were used to investigate relationships between cell volume and the carbon and nitrogen content of nondiatomaceous marine nanophytoplankton and found correlations were highly significant between biovolume of preserved cells and C and N contents.
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